Self-curing acrylic formulations with applications in intervertebral disk restoration:: drug release and biological behaviour

被引:7
作者
Larraz, Elena [1 ]
Elvira, Carlos [1 ]
Fernandez, Mar [2 ]
Parra, Juan [3 ]
Collia, Francisco [4 ]
Lopez-Bravo, Antonio [3 ]
Roman, Julio San [1 ]
机构
[1] CSIC, Inst Polymer Sci & Technol, Macromol Chem Dept, Madrid 28006, Spain
[2] UCM, Fac Pharm, Dept Pharmacol, Madrid 28040, Spain
[3] Avila Prov Hosp, Dept Anat Pathol, Avila 05003, Spain
[4] Univ Salamanca, Fac Med, Dept Human Anat & Histol, Salamanca 37006, Spain
关键词
intervertebral disks; injectable; drug release; acrylic; biocompatibility; surface analysis;
D O I
10.1002/term.10
中图分类号
Q813 [细胞工程];
学科分类号
摘要
New injectable acrylic formulations have been prepared to be applied in restoration processes for intervertebral disks (IUDs). The solid phase of the formulations is composed of poly(methyl methacrylate) (PMMA), incorporating in some cases chondroitin sulphate (CS) as a regenerative bioactive molecule, whereas the liquid phase is constituted by an amphiphilic macromonomer (MT), 2-hydroxyethyl methacrylate (HEMA) and, in some formulations, acrylic acid (AA). The curing parameters and the mechanical properties of the IVD formulations make them excellent candidates for intervertebral application. In vitro and in vivo evaluation of the prepared IVD formulations is described in terms of CS release, surface analysis after immersion in SBF solutions, and biocompatibility studies based on MTT assay and Alamar blue test, as well as in vivo implantation in female Wistar rats, by injection of the IVD formulations followed by histological evaluations to assess tissue response. Copyright (C) 2007 John Wiley & Sons, Ltd.
引用
收藏
页码:120 / 127
页数:8
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