In Vitro Cytotoxic Activity and Phytochemical Characterization (UPLC/T-TOF-MS/MS) of the Watermelon (Citrullus lanatus) Rind Extract

被引：9

作者：

El Gizawy, Heba A. ^{[1
]}

El-Haddad, Alaadin E. ^{[1
]}

Attia, Yasmin M. ^{[2
]}

Fahim, Sally A. ^{[3
]}

Zafer, Mai M. ^{[4
]}

Saadeldeen, Amr M. ^{[5
]}

机构：

[1] October 6 Univ, Dept Pharmacognosy, Fac Pharm, Giza 12585, Egypt

[2] Cairo Univ, Natl Canc Inst, Canc Biol Dept, Pharmacol Unit, Kasr Al Eini St, Cairo 11796, Egypt

[3] Newgiza Univ NGU, Sch Pharm, Dept Biochem, Km 22 Cairo Alexandria Desert Rd, Giza 12577, Egypt

[4] Ahram Canadian Univ, Fac Pharm, Microbiol & Immunol Dept, Giza 12566, Egypt

[5] Newgiza Univ NGU, Sch Pharm, Dept Pharmacognosy, Km 22 Cairo Alexandria Desert Rd, Giza 12577, Egypt

来源：

MOLECULES | 2022年 / 27卷 / 08期

关键词：

Citrullus lanatus; cancer cell lines; apoptosis; VEGF; cell cycle; MASS SPECTROMETRY; DIETARY FIBER; ANTIBACTERIAL; CONSTITUENTS; ANTIOXIDANTS; EXPRESSION; PHENOLICS; CUCUMBER; WASTE;

D O I：

10.3390/molecules27082480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reusing food waste is becoming popular in pharmaceutical industries. Watermelon (Citrullus lanatus) rind is commonly discarded as a major solid waste. Here, the in vitro cytotoxic potential of watermelon rind extracts was screened against a panel of human cancer cell lines. Cell cycle analysis was used to determine the induction of cell death, whereas annexin V-FITC binding, caspase-3, BAX, and BCL-2 mRNA expression levels were used to determine the degree of apoptosis. VEGF-promoting angiogenesis and cell migration were also evaluated. Moreover, the identification of phytoconstituents in the rind extract was achieved using UPLC/T-TOF-MS/MS, and a total of 45 bioactive compounds were detected, including phenolic acids, flavonoids aglycones, and their glycoside derivatives. The tested watermelon rind extracts suppressed cell proliferation in seven cancer cell lines in a concentration-dependent manner. The cytotoxicity of the rind aqueous extract (RAE) was higher compared with that of the other extracts. In addition to a substantial inhibitory effect on cell migration, the RAE triggered apoptosis in HCT116 and Hep2 cells by driving the accumulation of cells in the S phase and elevating the activity of caspase-3 and the BAX/BCL-2 ratio. Thus, a complete phytochemical and cytotoxic investigation of the Citrullus lanatus rind extract may identify its potential potency as an anticancer agent.

引用

页数：19