Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts

被引：1
|
作者：
Floy, Martha E. [1 ]
Shabnam, Fathima [1 ]
Palecek, Sean P. [1 ]
机构：
[1] Univ Wisconsin, Madison, WI 53706 USA
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 02期
关键词：
ANTIBIOTICS;
D O I：
10.1016/j.xpro.2022.101275
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cardiac fibroblasts (CFBs) are a key therapeutic target due to their supportive roles during heart development and response to injury and disease. Here, we describe a robust protocol to differentiate human pluripotent stem cells (hPSCs) into CFBs through an epicardial intermediate. We discuss in detail the characterization of the resulting epicardial-derived fibroblasts (EpiC-FBs) using immunofluorescence microscopy, flow cytometry, and qPCR. We anticipate that these EpiC-FBs can be applied to drug testing, disease modeling, and tissue engineering. For complete details on the use and execution of this protocol, please refer to Bao et al. (2016), Floy et al. (2021), and Lian et al. (2015).
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页数：38
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