Membrane-mediated transport in a non-equilibrium hybrid protocell based on coacervate droplets and a surfactant

被引:24
作者
Chang, Haojing [1 ]
Jing, Hairong [1 ]
Yin, Yudan [1 ]
Zhang, Qiufen [1 ]
Liang, Dehai [1 ]
机构
[1] Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Polymer Chem & Phys, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
基金
北京市自然科学基金; 中国国家自然科学基金;
关键词
ARTIFICIAL CELLS; VESICLES; TRANSFORMATION; MICRODROPLETS; ORGANELLES; GROWTH; MODELS; STEP;
D O I
10.1039/c8cc08337a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A hybrid protocell is constructed to investigate the membrane transport of neutral, cationic, and anionic molecules under non-equilibrium conditions. Each model molecule follows a specific pathway to be internalized and generates different distributions in the droplets. This work provides a step towards functionalization of synthetic protocells capable of biomimetic operations.
引用
收藏
页码:13849 / 13852
页数:4
相关论文
共 41 条
[1]  
Adamala K, 2013, NAT CHEM, V5, P495, DOI [10.1038/NCHEM.1650, 10.1038/nchem.1650]
[2]   RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly [J].
Aumiller, William M., Jr. ;
Cakmak, Fatma Pir ;
Davis, Bradley W. ;
Keating, Christine D. .
LANGMUIR, 2016, 32 (39) :10042-10053
[3]   Dilution of protein-surfactant complexes: A fluorescence study [J].
Azadi, Glareh ;
Chauhan, Anuj ;
Tripathi, Anubhav .
PROTEIN SCIENCE, 2013, 22 (09) :1258-1265
[4]   Growth and transformation of vesicles studied by ferritin labeling and cryotransmission electron microscopy [J].
Berclaz, N ;
Müller, M ;
Walde, P ;
Luisi, PL .
JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (05) :1056-1064
[5]   Progress Toward Synthetic Cells [J].
Blain, J. Craig ;
Szostak, Jack W. .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83, 2014, 83 :615-640
[6]  
Brangwynne CP, 2015, NAT PHYS, V11, P899, DOI [10.1038/NPHYS3532, 10.1038/nphys3532]
[7]   Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity [J].
Buddingh, Bastiaan C. ;
van Hest, Jan C. M. .
ACCOUNTS OF CHEMICAL RESEARCH, 2017, 50 (04) :769-777
[8]   From Self-Assembled Vesicles to Protocells [J].
Chen, Irene A. ;
Walde, Peter .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2010, 2 (07) :a002170
[9]   Chemical approaches to synthetic biology [J].
Chiarabelli, Cristiano ;
Stano, Pasquale ;
Luisi, Pier Luigi .
CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (04) :492-497
[10]   Microfluidic Formation of Monodisperse Coacervate Organelles in Liposomes [J].
Deng, Nan-Nan ;
Huck, Wilhelm T. S. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2017, 56 (33) :9736-9740