Onset of 310-helical secondary structure in aib oligopeptides probed by coherent 2D IR spectroscopy

被引:52
作者
Maekawa, Hiroaki [1 ]
Formaggio, Fernando [2 ]
Toniolo, Claudio [2 ]
Ge, Nien-Hui [1 ]
机构
[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA
[2] Univ Padua, Inst Biomol Chem, CNR, Padova Unit,Dept Chem, I-35131 Padua, Italy
关键词
D O I
10.1021/ja8007165
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have investigated the onset of the secondary structure and the evolution of two-dimensional infrared (2D IR) spectral patterns as a function of chain length with a study of 3(10)-helical peptides. The results show that 2D IR is highly sensitive to peptide conformation, disorder, and size. An extensive set of 2D IR spectra of C-alpha-methylated homopeptides, Z-(Aib)(n)-OtBu (n = 3, 5, 8, and 10), in CDC13 was measured in the amide-I region. The 2D spectral patterns of the tripeptide are quite different from those of the longer peptides. The spectral signatures begin to converge at the pentapeptide and become almost the same for the octa- and decapeptide. Simulations employing a vibrational exciton model were performed, with the local mode frequency shifts estimated from the intramolecular hydrogen bond electrostatic energies. The 2D spectra are well simulated using dihedral angle distributions around the average values (phi, psi) approximate to (-57 degrees, -31 degrees) with a width of similar to 21 degrees. The simulated site-dependent amide-I local mode frequencies are in agreement with those from scaled semiempirical AM1 calculations. The tripeptide exhibits a more noticeable discrepancy between the experimental and simulated cross-peak patterns. This behavior suggests the presence of a peptide population outside the single beta-turn conformation. The onset of the 3(10)-helical secondary structure appears to already occur at the pentapeptide level.
引用
收藏
页码:6556 / 6566
页数:11
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