Sensitivity and Specificity of a Loop-Mediated Isothermal Amplification Assay for the Detection of Mycoplasma Pneumonia from Nasopharyngeal Swab Samples Compared with those of Real-time PCR

被引：9

作者：

Ishiguro, Nobuhisa ^{[1
]}

Koseki, Naoko ^{[1
]}

Kaiho, Miki ^{[1
]}

Kikuta, Hideaki ^{[2
]}

Togashi, Takehiro ^{[3
]}

Watanabe, Toru ^{[4
]}

Ariga, Tadashi ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Med, Dept Pediat, Sapporo, Hokkaido 0608638, Japan

[2] Touei Hosp, Pediat Clin, Sapporo, Hokkaido, Japan

[3] Sapporo City Univ, Sch Nursing, Sapporo, Hokkaido, Japan

[4] Watanabe Pediat Allergy Clin, Sapporo, Hokkaido, Japan

来源：

CLINICAL LABORATORY | 2015年 / 61卷 / 5-6期

关键词：

loop-mediated isothermal amplification; real-time PCR; detection; Mycoplasma pneumonia; COMMUNITY-ACQUIRED PNEUMONIA; RAPID DIAGNOSIS; LAMP ASSAY; CHILDREN; INFECTION; SEROLOGY; UTILITY; DNA;

D O I：

10.7754/Clin.Lab.2014.141016

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The aim of this study was to determine the sensitivity and specificity of a loop-mediated isothermal amplification (LAMP) assay kit for the detection of Mycoplasma pneumonia (Eiken Chemical Co., Ltd, Tokyo, Japan) from nasopharyngeal swab samples compared with those of real-time PCR. Methods: Nasopharyngeal swab samples taken from 223 patients aged 3 - 18 years who were suspected of having respiratory tract infections associated with Mycoplasma pneumonia were used in this study. The samples were tested both by the LAMP assay and by real-time PCR for detection of Mycoplasma pneumonia. Results: The sensitivity and specificity of the LAMP assay for the detection of Mycoplasma pneumonia were 99.1% (105/106) and 100.0% (117/117), respectively. Conclusions: The LAMP assay for the detection of Mycoplasma pneumonia is an accurate and fast assay that is suitable as a diagnostic tool in the acute phase of Mycoplasma pneumonia infection.

引用

页码：603 / 606

页数：4

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