Real-time monitoring approach: Assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix

被引：18

作者：

Chen, Yu ^{[1
]}

Zhang, Juntao ^{[2
]}

Wang, Yanping ^{[1
]}

Zhang, Li ^{[1
]}

Julien, Reboud ^{[1
]}

Tang, Kumcheong ^{[1
]}

Balasubramanian, Narayanan ^{[1
]}

机构：

[1] Inst Microelect, Singapore 117685, Singapore

[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 09期

关键词：

real-time cell-on-chip system; in situ cell monitoring; NCI-H460; adhesion; collagen-coated electrodes; alpha; 2; beta; 1-integrin;

D O I：

10.1016/j.bios.2007.12.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper presents an in situ impedance chip system, which allows studying the effects of drugs on the behaviors of living cell in real-time. A new label-free measurement approach is introduced, which enables to assess the extent of the adhesion of a cell population to the extracellular matrix (ECM)-coated rnicroelectrode array, by adding together all the impedance changes from individually controlled microelectrodes. A high sensitivity was demonstrated and allowed us to monitor cell attachment with the resolution of a "few cells". The dose and pre-incubation time effects of antibodies against beta 1-integrin and its subunit alpha 2 beta 1-integrin on the adhesion behavior of NCI-H460 lung cancer cells to collagen type I was extensively studied. Results show that both anti-beta 1-integrin and anti-alpha 2 beta 1-integrin inhibit NCI-H460 cells attachment to collagen I. This indicates that beta 1-integrin is present on the surface of NCI-H460 cancer cells, and that its subunit alpha 2 beta 1 significantly affects NCI-H460 cells attachment on collagen I. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：1390 / 1396

页数：7

共 46 条

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