Unique translation initiation of mRNAs-containing TISU element

被引：83

作者：

Elfakess, Rofa ^{[1
]}

Sinvani, Hadar ^{[1
]}

Haimov, Ora ^{[1
]}

Svitkin, Yuri ^{[2
,3
]}

Sonenberg, Nahum ^{[2
,3
]}

Dikstein, Rivka ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[2] McGill Univ, Dept Biochem, Montreal, PQ H3A 1A3, Canada

[3] McGill Univ, Goodman Canc Ctr, Montreal, PQ H3A 1A3, Canada

来源：

NUCLEIC ACIDS RESEARCH | 2011年 / 39卷 / 17期

关键词：

START CODON RECOGNITION; 40S RIBOSOMAL-SUBUNIT; SECONDARY STRUCTURE; EUKARYOTIC RIBOSOMES; PROTEIN DHX29; MECHANISMS; COMPLEXES; EIF1; SELECTION; POSITION;

D O I：

10.1093/nar/gkr484

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translation Initiator of Short 5' UTR (TISU) is a unique regulatory element of both transcription and translation initiation. It is present in a sizable number of genes with basic cellular functions and a very short untranslated region (5' UTR). Here, we investigated translation initiation from short 5' UTR mRNAs with AUG in various contexts. Reducing 5' UTR length to the minimal functional size increases leaky scanning from weak and strong initiators but hardly affects translation initiation and ribosomal binding directed by TISU. Ribosome interaction with TISU mRNA is cap dependent and involves AUG downstream nucleotides that compensate for the absent 5' UTR contacts. Interestingly, eIF1 inhibits cap-proximal AUG selection within weak or strong contexts but not within TISU. Furthermore, TISU-directed translation is unaffected by inhibition of the RNA helicase eIF4A. Thus, TISU directs efficient cap-dependent translation initiation without scanning, a mechanism that would be advantageous when intracellular levels of eIF1 and eIF4A fluctuate.

引用

页码：7598 / 7609

页数：12

共 33 条

[1] Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo [J].

Cheung, Yuen-Nei ;

Maag, David ;

Mitchell, Sarah F. ;

Fekete, Christie A. ;

Algire, Mikkel A. ;

Takacs, Julie E. ;

Shirokikh, Nikolay ;

Pestova, Tatyana ;

Lorsch, Jon R. ;

Hinnebusch, Alan G. .

GENES & DEVELOPMENT, 2007, 21 (10) :1217-1230

[2] Identification of okadaic-acid-induced genes by mRNA differential display in glioma cells [J].

Chin, LS ;

Singh, SK ;

Wang, Q ;

Murray, SF .

JOURNAL OF BIOMEDICAL SCIENCE, 2000, 7 (02) :152-159

[3] TRANSFER RNA(IMET) FUNCTIONS IN DIRECTING THE SCANNING RIBOSOME TO THE START SITE OF TRANSLATION [J].

CIGAN, AM ;

FENG, L ;

DONAHUE, TF .

SCIENCE, 1988, 242 (4875) :93-97

[4] A Translation Initiation Element Specific to mRNAs with Very Short 5′UTR that Also Regulates Transcription [J].

Elfakess, Rofa ;

Dikstein, Rivka .

PLOS ONE, 2008, 3 (08)

[5] The mechanism of eukaryotic translation initiation and principles of its regulation [J].

Jackson, Richard J. ;

Hellen, Christopher U. T. ;

Pestova, Tatyana V. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2010, 11 (02) :113-127

[6] Coordinate regulation of ribosome biogenesis and function by the ribosomal protein S6 kinase, a key mediator of mTOR function [J].

Jastrzebski, Katarzyna ;

Hannan, Katherine M. ;

Tchoubrieva, Elissaveta B. ;

Hannan, Ross D. ;

Pearson, Richard B. .

GROWTH FACTORS, 2007, 25 (04) :209-226

[7] Regulation of translation via mRNA structure in prokaryotes and eukaryotes [J].

Kozak, M .

GENE, 2005, 361 :13-37

[8] Pushing the limits of the scanning mechanism for initiation of translation [J].

Kozak, M .

GENE, 2002, 299 (1-2) :1-34

[9]

KOZAK M, 1991, GENE EXPRESSION, V1, P117

[10] DOWNSTREAM SECONDARY STRUCTURE FACILITATES RECOGNITION OF INITIATOR CODONS BY EUKARYOTIC RIBOSOMES [J].

KOZAK, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8301-8305

← 1 2 3 4 →