Halomonas lactosivorans sp. nov., isolated from salt-lake sediment

A bacteria strain, designated CFH 90008(T) was isolated from a salt lake sediment sample collected from Yuncheng city, Shanxi Province, PR China. Strain CFH 90008(T) was Gram-stain-negative, strictly aerobic, motile with lateral flagella and rod-shaped. Colonies were yellow, circular and smooth. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CFH 90008(T) belonged to the genus Halomonas, showing highest sequence similarity to Halomonas daqingensis DQD2-30(T) (98.6%). Halomonas saliphila LCB169(T) (98.5%), Halomonas desiderata FB2(T) (98.1%) and Halomonas kenyensis AIR 2(T) (98.0%). Good growth was observed at 10-50 degrees C, pH 6.0-9.0 and with NaCl concentration from 1.0 to 12.0% (w/v). The predominant quinone was Q9. The major fatty acid (>10%) was C-18(:1) omega 7c, C-16:0 and C-16:1 omega 7c. The genome of strain CFH 90008(T) was 4.36 Mbp with a genomic DNA G+C content of 66.7 mol%. Based on low average nucleotide identity and DNA-DNAhybridization results, chemotaxonomic characteristics, and differential physiological properties, strain CFH 90008(T) could not be classified into any recognized species of the genus Halomonas. Therefore, a new species, for which the name Halomonas lactosivorans sp. nov. is proposed. The type strain is CFH 90008(T) (=DSM 103220(T)=KCTC 52281(T)).