Fecal Progestagens to Detect and Monitor Pregnancy in Captive Female Cheetahs (Acinonyx jubatus)

被引：6

作者：

Adachi, Itsuki ^{[2
]}

Kusuda, Satoshi ^{[1
]}

Kawai, Hitomi ^{[1
]}

Ohazama, Megumi ^{[3
]}

Taniguchi, Atsushi ^{[4
]}

Kondo, Natsuko ^{[4
]}

Yoshihara, Masato ^{[4
]}

Okuda, Ryuta ^{[5
]}

Ishikawa, Tatsuya ^{[5
]}

Kanda, Iwai ^{[6
]}

Doi, Osamu ^{[1
]}

机构：

[1] Gifu Univ, Fac Appl Biol Sci, Gifu 5011193, Japan

[2] Gifu Univ, Grad Sch Agr Sci, Gifu 5011193, Japan

[3] Himeji Cent Pk, Himeji, Hyogo 6792121, Japan

[4] Tama Zool Pk, Tokyo 1910042, Japan

[5] Fuji Safari Pk, Shizuoka 4101231, Japan

[6] Kyushu Nat Zool Pk, Oita 8720722, Japan

来源：

JOURNAL OF REPRODUCTION AND DEVELOPMENT | 2011年 / 57卷 / 02期

基金：

日本学术振兴会;

关键词：

Cheetah; Fecal hormone; Progesterone; Steroid metabolites; ESTRADIOL-17-BETA; METABOLISM; FECES;

D O I：

10.1262/jrd.10-057T

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The purposes of the present study were to establish a noninvasive monitoring assay of fecal progestagen measurement to detect pregnancy and to identify the components of fecal progestagens in early, middle and late pregnancy in cheetahs. Feces were collected from 7 female cheetahs and analyzed from 30 days before the last copulation to parturition in 9 pregnancies. Blood was collected from one cheetah. Fecal progestagen and serum progesterone concentrations were determined by enzyme immunoassay (EIA). The profiles of the fecal progestagen concentrations were similar to the serum progesterone profile. Fecal progestagen and serum progesterone concentrations remained at the baseline until copulation. In the mean fecal progestagen profile during pregnancy (92.8 +/- 0.4 days; from the last copulation to parturition), the concentrations increased 3-4 days after the last copulation and remained high until parturition. To investigate changes in the components of progestagen metabolites in the tripartite periods of gestation, fecal progestagens were analyzed by HPLC-EIA. Marked immunoreactive peaks consistent with 5 alpha-pregnan-3 alpha/beta-ol-20-one and 5 alpha-pregnan-3,20-dione and small peaks consistent with 5 beta-pregnan-3 alpha/beta-ol-20-one were detected. There were no distinct difference in the components of progestagens among the first, second and third trimesters of pregnancy. The hormone assay, as an indicator of fecal 5 alpha-reduced pregnanes, is useful for detecting pregnancy and monitoring pregnant luteal activity in cheetahs.

引用

页码：262 / 266

页数：5