Glutathione-dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H2O2

被引:30
作者
Zhang, Tianru [1 ,2 ]
Ma, Mingyue [1 ,2 ]
Chen, Tao [1 ,2 ]
Zhang, Linlin [1 ,2 ]
Fan, Lingling [1 ,2 ]
Zhang, Wei [1 ,2 ]
Wei, Bo [1 ,2 ]
Li, Shengchun [3 ]
Xuan, Wei [4 ,5 ]
Noctor, Graham [6 ,7 ]
Han, Yi [1 ,2 ]
机构
[1] Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China
[2] Hefei Univ Technol, Engn Res Ctr Bioproc, Minist Educ, Hefei, Peoples R China
[3] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Peoples R China
[4] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China
[5] Nanjing Agr Univ, MOA Key Lab Plant Nutr & Fertilizat Lower Middle, Nanjing, Peoples R China
[6] Univ Paris Saclay, Sorbonne Paris Cite, Inst Plant Sci Paris Saclay IPS2, CNRS,INRA,Univ Evry,Paris Diderot, Orsay, France
[7] Inst Univ France, Paris, France
关键词
denitrosation; glutathione; GSNOR1; H2O2; oxidative stress; salicylic acid; S-NITROSOGLUTATHIONE REDUCTASE; GENE-EXPRESSION; NITRIC-OXIDE; CELL-DEATH; HYDROGEN-PEROXIDE; SALICYLIC-ACID; ARABIDOPSIS MUTANTS; REDOX REGULATION; PATHOGENESIS RESPONSES; HIGH LIGHT;
D O I
10.1111/pce.13727
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Photorespiratory hydrogen peroxide (H2O2) plays key roles in pathogenesis responses by triggering the salicylic acid (SA) pathway in Arabidopsis. However, factors linking intracellular H2O2 to activation of the SA pathway remain elusive. In this work, the catalase-deficient Arabidopsis mutant, cat2, was exploited to elucidate the impact of S-nitrosoglutathione reductase 1 (GSNOR1) on H2O2-dependent signalling pathways. Introducing the gsnor1-3 mutation into the cat2 background increased S-nitrosothiol levels and abolished cat2-triggered cell death, SA accumulation, and associated gene expression but had little additional effect on the major components of the ascorbate-glutathione system or glycolate oxidase activities. Differential transcriptome profiles between gsnor1-3 and cat2 gsnor1-3 together with damped ROS-triggered gene expression in cat2 gsnor1-3 further indicated that GSNOR1 acts to mediate the SA pathway downstream of H2O2. Up-regulation of GSNOR activity was compromised in cat2 cad2 and cat2 pad2 mutants in which glutathione accumulation was genetically prevented. Experiments with purified recombinant GSNOR revealed that the enzyme is posttranslationally regulated by direct denitrosation in a glutathione-dependent manner. Together, our findings identify GSNOR1-controlled nitrosation as a key factor in activation of the SA pathway by H2O2 and reveal that glutathione is required to maintain this biological function.
引用
收藏
页码:1175 / 1191
页数:17
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