Blueberry extract attenuates γ-radiation-induced hepatocyte damage by modulating oxidative stress and suppressing NF-κB in male rats

被引:18
作者
Alkhalf, Maha, I [1 ,2 ]
Khalifa, Fares K. [3 ,4 ]
机构
[1] King Abdulaziz Univ, Biochem Dept, Fac Sci, Jeddah, Saudi Arabia
[2] Univ Jeddah, Appl Biochem Dept, Fac Sci, AL Faisaliah Campus, Jeddah, Saudi Arabia
[3] King Abdulaziz Univ, Biochem Dept, Sci Fac Alsolimania, Jeddah, Saudi Arabia
[4] Ain Shams Univ, Biochem & Nutr Dept, Women Coll, Cairo, Egypt
关键词
gamma-Irradiation-blueberry-hepatic NF-kappa B; Cytokines-DNA fragmentation; ANTHOCYANINS; IRRADIATION; EXPRESSION; INJURY; CELLS; LIVER; MICE;
D O I
10.1016/j.sjbs.2018.07.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Radiation exposure is known to produce many harmful effects in biological systems, and these effects are often mediated by oxygen free radicals. Because blueberries are rich in antioxidant compounds such as anthocyanins and phenolic acids, we divided forty adult rats into four treatment groups of 10 (G1-4) as follows: G1 rats were used as a control, G2 rats were irradiated with 8 Gy at 2 Gy/week at a dose rate of 0.5 Gy/min, G3 rats were administered blueberry extract (200 mg/kg) and G4 rats were administered blueberry extract during the same irradiation period. In subsequent determinations, gamma-irradiated rats had increased levels of cholesterol, triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL), and significantly elevated liver enzyme activities, including alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), and total bilirubin. In contrast, significant reductions in albumin, total protein and globulin were observed, whereas gamma irradiation decreased activities of the antioxidant enzymes glutathione (GSH), catalase (CAT), xanthine dehydrogenase (XDH) and superoxide dismutase (SOD). We also observed incremental increases in DNA fragmentation percentages and histopathological changes in liver tissues. Serum pro-inflammatory cytokine levels (IL-6, IL-10 and TNF-alpha) were significantly elevated and hepatic NF-kappa B was upregulated. In G4 rats, treatments with blueberry extract restored liver pro-oxidant status, reduced cytokine levels, ameliorated histopathological parameters and reduced DNA damage. In conclusion, gamma-radiation exerts toxic effects in the rat livers, and blueberry extract is protective against these. (C) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
引用
收藏
页码:1272 / 1277
页数:6
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