A novel, efficient regenerating method of NADPH using a new formate dehydrogenase

被引：110
作者：
Seelbach, K
Riebel, B
Hummel, W
Kula, MR
Tishkov, VI
Egorov, AM
Wandrey, C
Kragl, U
机构：
[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL, D-52425 JULICH, GERMANY
[2] UNIV DUSSELDORF, FORSCH ZENTRUM JULICH GMBH, INST ENZYMTECHNOL, D-52404 JULICH, GERMANY
[3] MOSCOW MV LOMONOSOV STATE UNIV, FAC CHEM, DEPT CHEM ENZYMOL, MOSCOW 119899, RUSSIA
来源：
TETRAHEDRON LETTERS | 1996年 / 37卷 / 09期
关键词：
D O I：
10.1016/0040-4039(96)00010-X
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis viith other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.
引用
收藏
页码：1377 / 1380
页数：4
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