Immobilization of α-glucosidase in chitosan coated polygalacturonic acid

被引：13

作者：

Dinçer, A ^{[1
]}

Okutucu, B ^{[1
]}

Zihnioglu, F ^{[1
]}

Telefoncu, A ^{[1
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2005年 / 35卷 / 02期

关键词：

alpha-glucosidase; chitosan coated polygalacturonic acid (PGA) beads; immobilization;

D O I：

10.1081/PB-200054695

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crude alpha-glucosidase from Baker's yeast was immobilized in polygalacturonic acid beads and coated with chitosan. Chemical and physical characterization were performed by using p-nitrophenyl-alpha-D-glucopyranoside (pNPG) as an artificial substrate. Operation, thermal, pH, and strorage stabilities of the free and immobilized enzyme were also examined. The stabilities of immobilized enzyme were found to be better than that of the free enzyme. Furthermore, the hydrolysis rate of the chitosan coated alpha-glucosidase polygalacturonic acid beads were studied. In conclusion, the enzyme beads appear to have good characteristics and offer the prospect that this system may find application in enzyme immobilization, in addition to controlled drug release studies.

引用

页码：103 / 111

页数：9

共 15 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] An experiment illustrating the effect of immobilisation on enzyme properties [J].

Busto, MD .

BIOCHEMICAL EDUCATION, 1998, 26 (04) :304-308

[3] Pharmaceutical applications of chitosan [J].

Dodane, V ;

Vilivalam, VD .

PHARMACEUTICAL SCIENCE & TECHNOLOGY TODAY, 1998, 1 (06) :246-253

[4] Clinical and metabolic correction of Pompe disease by enzyme therapy in acid maltase-deficient quail [J].

Kikuchi, T ;

Yang, HW ;

Pennybacker, M ;

Ichihara, N ;

Mizutani, M ;

Van Hove, JLK ;

Chen, YT .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (04) :827-833

[5] Release of albumin from chitosan-coated pectin beads in vitro [J].

Kim, TH ;

Park, YH ;

Kim, KJ ;

Cho, CS .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2003, 250 (02) :371-383

[6]

Kumar MNVR, 2000, J PHARM PHARM SCI, V3, P234

[7] Pectin-based systems for colon-specific drug delivery via oral route [J].

Liu, LS ;

Fishman, ML ;

Kost, J ;

Hicks, KB .

BIOMATERIALS, 2003, 24 (19) :3333-3343

[8] A PRACTICAL EXPERIMENT ON ENZYME IMMOBILIZATION AND CHARACTERIZATION OF THE IMMOBILIZED DERIVATIVES [J].

MANJON, A ;

IBORRA, JL ;

LOZANO, P ;

CANOVAS, M .

BIOCHEMICAL EDUCATION, 1995, 23 (04) :213-216

[9] In vitro survey of alpha-glucosidase inhibitory food components [J].

Matsui, T ;

Yoshimoto, C ;

Osajima, K ;

Oki, T ;

Osajima, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (12) :2019-2022

[10] IMMOBILIZATION OF INVERTASE USING A RECYCLING ENZYME REACTOR SYSTEM [J].

SCHNEIDER, F ;

MARCZAK, R .

BIOCHEMICAL EDUCATION, 1991, 19 (02) :83-85

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