Objective To determine the effectiveness and incidence of complications of palmar digital neurectomy using the guillotine technique in horses presented for lameness originating from sites innervated by these nerves. Design Retrospective study of 24 clinical cases. Animals Twenty-four horses undergoing a palmar digital nerve neurectomy between June 1997 and June 2001. Methods The records of all horses undergoing a uniaxial or biaxial, unilateral or bilateral neurectomy during the defined time period were retrieved. Information retrieved from the record included breed, age, gender, discipline for which the horse was used, history, results of lameness examination, results of perineural anaesthesia, radiographic findings, surgery report, postoperative care, complications and outcome. Results Seventeen geldings and seven female horses of mixed breeds, ranging in age from 1 to 16 years, that were used for a variety of non-racing, but competitive, athletic disciplines, underwent neurectomy using the guillotine technique during the specified time period. In 23 horses perineural anaesthesia was performed at the University Veterinary Centre to isolate the lameness. Anaesthesia resolved lameness in 18 horses. In five horses there was some residual lameness isolated to sites remote from the distal extremity. The most common radiographic finding was an increase in the number and or size of the synovial fossae of the navicular bone. Twenty-two horses underwent bilateral biaxial neurectomies and two horses with painful trauma-induced neuromas underwent unilateral, uniaxial neurectomy. There were no postoperative complications. All horses were rested for 3 months before returning to work. At least 1 year after surgery, 22 horses were in full work and two horses were not in work because of unrelated problems. Of the horses in work, 17 were considered free of lameness. The other five were competing, but receiving medication for residual lameness associated with sites remote from the distal extremity. Conclusion Results of this study suggested that palmar digital neurectomy using the guillotine technique provided reliable and consistent resolution of lameness originating from sites innervated by these nerves. There were no significant complications.