Diurnal protein oscillation profiles in Drosophila head

被引：5
作者：
Du, Juan [1 ]
Zhang, Yifan [1 ]
Xue, Yongbo [2 ]
Zhao, Xiaoyun [3 ]
Zhao, Xianguo [1 ]
Wei, Yu [1 ]
Li, Zhen [3 ]
Zhang, Yong [2 ]
Zhao, Zhangwu [1 ]
机构：
[1] China Agr Univ, Coll Plant Protect, Dept Entomol, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China
[2] Univ Nevada, Dept Biol, Reno, NV 89557 USA
[3] China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China
来源：
FEBS LETTERS | 2018年 / 592卷 / 22期
基金：
中国国家自然科学基金; 美国国家卫生研究院;
关键词：
circadian rhythm; clock; Drosophila; proteomics; TRANSCRIPTIONAL REPRESSOR; CLOCKWORK-ORANGE; DOUBLE-TIME; O-GLCNACYLATION; NUCLEAR ENTRY; GENE; PERIOD; PHOSPHORYLATION; REVEALS; ENCODES;
D O I：
10.1002/1873-3468.13267
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome-wide analysis of protein oscillations is still lacking in Drosophila. In this study, the total protein and phosphorylated protein in Drosophila heads in a 24-h daily time-course were assayed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and 10 and 7 oscillating proteins as well as 19 and 22 oscillating phosphoproteins in the w(1118) control and Clk(Jrk) mutant strains were separately identified. Lastly, we performed a mini screen to investigate the functions of some oscillating proteins in circadian locomotion rhythms. This study provides the first proteomic profiling of diurnally oscillating proteins in fly heads, thereby providing a basis for further mechanistic studies of these proteins in circadian rhythm.
引用
收藏
页码：3736 / 3749
页数：14
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