Diurnal protein oscillation profiles in Drosophila head

被引：5

作者：

Du, Juan ^{[1
]}

Zhang, Yifan ^{[1
]}

Xue, Yongbo ^{[2
]}

Zhao, Xiaoyun ^{[3
]}

Zhao, Xianguo ^{[1
]}

Wei, Yu ^{[1
]}

Li, Zhen ^{[3
]}

Zhang, Yong ^{[2
]}

Zhao, Zhangwu ^{[1
]}

机构：

[1] China Agr Univ, Coll Plant Protect, Dept Entomol, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

[2] Univ Nevada, Dept Biol, Reno, NV 89557 USA

[3] China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China

来源：

FEBS LETTERS | 2018年 / 592卷 / 22期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

circadian rhythm; clock; Drosophila; proteomics; TRANSCRIPTIONAL REPRESSOR; CLOCKWORK-ORANGE; DOUBLE-TIME; O-GLCNACYLATION; NUCLEAR ENTRY; GENE; PERIOD; PHOSPHORYLATION; REVEALS; ENCODES;

D O I：

10.1002/1873-3468.13267

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome-wide analysis of protein oscillations is still lacking in Drosophila. In this study, the total protein and phosphorylated protein in Drosophila heads in a 24-h daily time-course were assayed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and 10 and 7 oscillating proteins as well as 19 and 22 oscillating phosphoproteins in the w(1118) control and Clk(Jrk) mutant strains were separately identified. Lastly, we performed a mini screen to investigate the functions of some oscillating proteins in circadian locomotion rhythms. This study provides the first proteomic profiling of diurnally oscillating proteins in fly heads, thereby providing a basis for further mechanistic studies of these proteins in circadian rhythm.

引用

页码：3736 / 3749

页数：14

共 46 条

[1] RNA-seq analysis of Drosophila clock and non-clock neurons reveals neuron-specific cycling and novel candidate neuropeptides [J].

Abruzzi, Katharine C. ;

Zadina, Abigail ;

Luo, Weifei ;

Wiyanto, Evelyn ;

Rahman, Reazur ;

Guo, Fang ;

Shafer, Orie ;

Rosbash, Michael .

PLOS GENETICS, 2017, 13 (02)

[2]

Abruzzi KC, 2011, GENE DEV, V25, P2374, DOI [10.1101/gad.178079.111, 10.1101/gad.174110.111]

[3] A role for CK2 in the Drosophila circadian oscillator [J].

Akten, B ;

Jauch, E ;

Genova, GK ;

Kim, EY ;

Edery, I ;

Raabe, T ;

Jackson, FR .

NATURE NEUROSCIENCE, 2003, 6 (03) :251-257

[4] A mutant Drosophila homolog of mammalian Clock disrupts circadian rhythms and transcription of period and timeless [J].

Allada, R ;

White, NE ;

So, WV ;

Hall, JC ;

Rosbash, M .

CELL, 1998, 93 (05) :791-804

[5] The Proteomic Landscape of the Suprachiasmatic Nucleus Clock Reveals Large-Scale Coordination of Key Biological Processes [J].

Chiang, Cheng-Kang ;

Mehta, Neel ;

Patel, Abhilasha ;

Zhang, Peng ;

Ning, Zhibin ;

Mayne, Janice ;

Sun, Warren Y. L. ;

Cheng, Hai-Ying M. ;

Figeys, Daniel .

PLOS GENETICS, 2014, 10 (10)

[6] NEMO/NLK Phosphorylates PERIOD to Initiate a Time-Delay Phosphorylation Circuit that Sets Circadian Clock Speed [J].

Chiu, Joanna C. ;

Ko, Hyuk Wan ;

Edery, Isaac .

CELL, 2011, 145 (03) :357-370

[7] Circadian regulation of gene expression systems in the Drosophila head [J].

Claridge-Chang, A ;

Wijnen, H ;

Naef, F ;

Boothroyd, C ;

Rajewsky, N ;

Young, MW .

NEURON, 2001, 32 (04) :657-671

[8] Interactive Features of Proteins Composing Eukaryotic Circadian Clocks [J].

Crane, Brian R. ;

Young, Michael W. .

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83, 2014, 83 :191-219

[9] The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period [J].

Cyran, SA ;

Yiannoulos, G ;

Buchsbaum, AM ;

Saez, L ;

Young, MW ;

Blau, J .

JOURNAL OF NEUROSCIENCE, 2005, 25 (22) :5430-5437

[10] Proteomic Analysis Reveals the Role of Synaptic Vesicle Cycling in Sustaining the Suprachiasmatic Circadian Clock [J].

Deery, Michael J. ;

Maywood, Elizabeth S. ;

Chesham, Johanna E. ;

Sladek, Martin ;

Karp, Natasha A. ;

Green, Edward W. ;

Charles, Philip D. ;

Reddy, Akhilesh B. ;

Kyriacou, Charalambos P. ;

Lilley, Kathryn S. ;

Hastings, Michael H. .

CURRENT BIOLOGY, 2009, 19 (23) :2031-2036

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