Propacin, a coumarinolignoid isolated from durian, inhibits the lipopolysaccharide-induced inflammatory response in macrophages through the MAPK and NF-κB pathways

被引：31

作者：

Huang, Yuying ^{[1
]}

Wang, Yihai ^{[1
,2
]}

Xu, Jingwen ^{[1
,2
]}

Feng, Jianying ^{[1
]}

He, Xiangjiu ^{[1
,2
]}

机构：

[1] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China

[2] Guangdong Engn Res Ctr Lead Cpds & Drug Discovery, Guangzhou 510006, Peoples R China

来源：

FOOD & FUNCTION | 2020年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

SIGNALING PATHWAY; SUPPRESSION; ACTIVATION; CELLS;

D O I：

10.1039/c9fo02202c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Durian, known as the king of fruits, is rich in nutrients and bioactive phytochemicals. Propacin is a bioactive coumarinolignoid isolated from durian. In this study, we demonstrated its anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW264.7 cells and explored the underlying mechanism. Propacin suppressed nitric oxide (NO) and prostaglandin E2 (PGE(2)) production in LPS-stimulated macrophages significantly by downregulating the mRNA and protein expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, propacin decreased the overexpression of the LPS-induced reactive oxygen species (ROS) and maintained the mitochondrial integrity in active macrophages. Furthermore, propacin inhibited the translocation of the nuclear factor-kappa B (NF-kappa B) p65 subunit into the nucleus and the phosphorylation of mitogen-activated protein kinases (MAPKs), especially JNK and ERK. Collectively, these data indicated that propacin may have the potential to be developed as a novel therapeutic agent for inflammatory-related diseases.

引用

页码：596 / 605

页数：10

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