Discrimination between receptor- and store-operated Ca2+ influx in human neutrophils

被引：8
|
作者：
Salmon, Michael D. [1 ]
Ahluwalia, Jatinder [1 ]
机构：
[1] Univ E London, Leukocyte & Ion Channel Res Lab, Sch Hlth & Biosci, London E15 4LZ, England
来源：
CELLULAR IMMUNOLOGY | 2010年 / 265卷 / 01期
关键词：
Calcium; Strontium; 2-APB; Pyr3; Neutrophils; 2-AMINOETHOXYDIPHENYL BORATE; 2-AMINOETHYLDIPHENYL BORATE; TRPC3; CHANNELS; CALCIUM-ENTRY; INHIBITION; EXOCYTOSIS; OCCURS;
D O I：
10.1016/j.cellimm.2010.07.009
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Ca2+ and Sr2+ entry pathways activated by pro-inflammatory agonists FMLP, LTB4 and PAF have been compared to thapsigargin in human neutrophils. 2-APB (10 mu M) increased Ca2+ influx and to a greater extent in agonist than in thapsigargin stimulated neutrophils. This action of 2-APB was specific to Ca2+ because 2-APB did not augment Sr2+ entry in agonist and thapsigargin stimulated neutrophils. This suggests that Ca2+ and Sr2+ entry can be used to discriminate between receptor and non-receptor (store)operated Ca2+ influx. Our data show for the first time that Pyr3 whilst partially inhibiting agonist induced Ca2+ influx almost completely abolished Ca2+ influx after thapsigargin stimulation. (C) 2010 Elsevier Inc. All rights reserved.
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页码：1 / 5
页数：5
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