Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani

被引：10

作者：

Long, John J. ^{[1
,2
]}

Luna, Emily K. ^{[2
]}

Jackson, Mary ^{[3
]}

Wheat, William ^{[3
]}

Jahn, Courtney E. ^{[2
]}

Leach, Jan E. ^{[2
]}

机构：

[1] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA

[2] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA

[3] Colorado State Univ, Dept Microbiol Immunol & Pathol, Mycobacteria Res Labs, Ft Collins, CO 80523 USA

来源：

BMC RESEARCH NOTES | 2019年 / 12卷 / 01期

关键词：

Acanthamoeba; Dictyostelium; Free-living amoebae; Rhizoctonia solani; Sheath blight; Vermamoeba;

D O I：

10.1186/s13104-019-4802-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ObjectiveRhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mechanisms in rice plants have not been identified. We hypothesize that alternate means of control are available in the environment including free-living amoebae. Amoebae are soil-, water- and air-borne microorganisms that are predominantly heterotrophic. Many amoeba species are mycophagous, and several harm their prey using mechanisms other than phagocytosis. Here, we used light and scanning electron microscopy to survey the interactions of R. solani with four amoeba species, with the goal of identifying amoebae species with potential for biocontrol.ResultsWe observed a wide range of responses during interactions of R. solani with four different free-living amoebae. Two Acanthamoeba species encyst in co-cultures with R. solani at higher rates than medium without R. solani. Vermamoeba vermiformis (formerly Hartmanella vermiformis) attach to R. solani mycelium and are associated with mycelial shriveling and perforations of fungal cell walls, indicating an antagonistic interaction. No phenotypic changes were observed in co-cultures of Dictyostelium discoideum and R. solani.

引用

页数：7

共 16 条

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