A molecular tool for genetic surveys in the red coral (Corallium rubrum):: An Inter-Simple Sequence Repeats (ISSRs) perspective

被引：5
|
作者：
Casu, M. [1 ]
Casu, D. [2 ]
Lai, T. [1 ]
Cossu, P. [1 ]
Curini-Galletti, M. [1 ]
机构：
[1] Univ Sassari, Dipartimento Zool & Genet Evoluzionist, I-07100 Sassari, Italy
[2] Univ Sassari, Dipartimento Bot & Ecol Vegetale, I-07100 Sassari, Italy
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2008年 / 36卷 / 02期
关键词：
Corallium rubrum; harvested species; molecular tool; Inter-Simple Sequence Repeat; genetic survey;
D O I：
10.1016/j.bse.2007.08.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Corallium rubrum is a colonial cnidarian, distributed in Mediterranean and Atlantic rocky bottoms. The species has been largely exploited for use in jewellery since 17th century, raising increasing concerns for the sustainability of the present rate of harvesting. The aim of this study was to standardise a simple and not expensive molecular technique to perforin genetic analysis oil populations of C. rubrum, in order to provide a useful tool for future surveys. Twelve samples collected from one shallow-water and two deep sites located at the Capo Caccia-Isola Piana MPA (North-western Sardinia, Italy) were surveyed by means of 10 ISSR (Inter-Simple Sequence Repeat) primers. Our results confirmed the ease of use and the reliability of ISSR markers, and their usefulness in detecting genetic variability even among individuals. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：77 / 83
页数：7
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