Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body

被引：42
作者：
Tsao, Che-Chia [1 ]
Gorovsky, Martin A. [1 ]
机构：
[1] Univ Rochester, Dept Biol, Rochester, NY 14627 USA
来源：
JOURNAL OF CELL SCIENCE | 2008年 / 121卷 / 04期
关键词：
intraflagellar transport; cilia/flagella; ciliary assembly; WD domain protein; gene targeting;
D O I：
10.1242/jcs.015826
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Intraflagellar transport (IFT) moves multiple protein particles composed of two biochemically distinct complexes, IFT-A and IFT-B, bi-directionally within cilia and is essential for cilia assembly and maintenance. We identified an ORF from the Tetrahymena macronuclear genome sequence, encoding IFT122A, an ortholog of an IFT-A complex protein. Tetrahymena IFT122A is induced during cilia regeneration, and epitope-tagged Ift122Ap could be detected in isolated cilia. IFT122A knockout cells still assembled cilia, albeit with lower efficiency, and could regenerate amputated cilia. Ift172p and Ift88p, two IFT-B complex proteins that localized mainly to basal bodies and along the cilia in wild-type cells, became preferentially enriched at the ciliary tips in IFT122A knockout cells. Our results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body.
引用
收藏
页码：428 / 436
页数：9
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