Platelet procoagulant potential is reduced in platelet concentrates ex vivo but appears restored following transfusion

被引:9
作者
Tohidi-Esfahani, Ibrahim [1 ,2 ,3 ]
Tan, Shereen [4 ]
Tan, Chuen Wen [1 ,5 ]
Johnson, Lacey [4 ]
Marks, Denese C. [3 ,4 ]
Chen, Vivien M. [1 ,2 ,3 ]
机构
[1] Univ Sydney, ANZAC Res Inst, Sydney, NSW, Australia
[2] Concord Repatriat Gen Hosp, Haematol Dept, Sydney, NSW, Australia
[3] Univ Sydney, Fac Med & Hlth, Sydney Med Sch, Sydney, NSW, Australia
[4] Australian Red Cross Lifeblood, Res & Dev, Sydney, NSW, Australia
[5] Singapore Gen Hosp, Haematol Dept, Singapore, Singapore
关键词
cryopreserved platelets; platelet concentrates; procoagulant platelets; storage lesion; thrombin generation; transfusion; CRYOPRESERVED PLATELETS; FLOW-CYTOMETRY; STORAGE; PHOSPHATIDYLSERINE; ACTIVATION; APHERESIS; RECOVERY; MARKERS;
D O I
10.1111/trf.16695
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background The procoagulant profile of platelet concentrates (PCs) following transfusion has been difficult to evaluate due to lack of specific markers. This study aimed to characterize procoagulant platelets in PCs and the effect of transfusion. Study design and methods Buffy coat-derived PCs from 12 donors were pooled, split, then stored conventionally, cold (2-6 degrees C) or cryopreserved (-80 degrees C). Procoagulant platelet profiles were assessed by flow cytometry (GSAO(+)/P-selectin(+)), lactadherin-binding, and calibrated automated thrombogram, during storage, unstimulated, or after thrombin and collagen stimulation and compared with blood from healthy volunteers. Platelet activation (P-selectin) and procoagulant platelet formation potential were measured (flow cytometry) in patients receiving clinically indicated conventional PC transfusion. Results Independent of significant increases with storage, procoagulant platelet proportions with and without agonist stimulation were significantly blunted in conventionally stored PCs (stimulated day 5 conventional PC 4.2 +/- 1.3%, healthy volunteer blood 11.1 +/- 2.9%; p < .0001). Cryopreserved PCs contained the highest proportion of procoagulant platelets (unstimulated: cryopreserved 25.6 +/- 1.8% vs. day 5 conventional 0.5 +/- 0.1% vs. day 14 cold-stored 5.8 +/- 1.0%, p < .0001), but demonstrated minimal increase with agonist. Transfusion of PCs was associated with an increase in procoagulant platelets (2.2 +/- 1.4% vs. 0.6 +/- 0.2%; p = .004) and reversal of the blunted agonist response (15.8 +/- 5.9% vs. 4.0 +/- 1.6%; p < .0001). Procoagulant responses post-transfusion were significantly higher than healthy controls, suggesting a priming effect. The P-selectin agonist response was not restored upon transfusion (79.4 +/- 13.9% vs. 82.0 +/- 2.5%). Conclusion Storage blunts the procoagulant platelet response to agonist stimulation in PCs. Despite this, conventionally stored PCs have high procoagulant potential following transfusion, with a discordant, persistent reduction in P-selectin response.
引用
收藏
页码:3420 / 3431
页数:12
相关论文
共 41 条
[31]   Impact of cold storage on platelets treated with Intercept pathogen inactivation [J].
Six, Katrijn R. ;
Devloo, Rosalie ;
Compernolle, Veerle ;
Feys, Hendrik B. .
TRANSFUSION, 2019, 59 (08) :2662-2671
[32]   Comparison between manufacturing sites shows differential adhesion, activation, and GPIbα expression of cryopreserved platelets [J].
Six, Katrijn R. ;
Delabie, Willem ;
Devreese, Katrien M. J. ;
Johnson, Lacey ;
Marks, Denese C. ;
Dumont, Larry J. ;
Compernolle, Veerle ;
Feys, Hendrik B. .
TRANSFUSION, 2018, 58 (11) :2645-2656
[33]   Platelet subpopulations remain despite strong dual agonist stimulation and can be characterised using a novel six-colour flow cytometry protocol [J].
Sodergren, Anna Linnea ;
Ramstrom, Sofia .
SCIENTIFIC REPORTS, 2018, 8
[34]   A No-Prophylaxis Platelet-Transfusion Strategy for Hematologic Cancers [J].
Stanworth, Simon J. ;
Estcourt, Lise J. ;
Powter, Gillian ;
Kahan, Brennan C. ;
Dyer, Claire ;
Choo, Louise ;
Bakrania, Lekha ;
Llewelyn, Charlotte ;
Littlewood, Timothy ;
Soutar, Richard ;
Norfolk, Derek ;
Copplestone, Adrian ;
Smith, Neil ;
Kerr, Paul ;
Jones, Gail ;
Raj, Kavita ;
Westerman, David A. ;
Szer, Jeffrey ;
Jackson, Nicholas ;
Bardy, Peter G. ;
Plews, Dianne ;
Lyons, Simon ;
Bielby, Linley ;
Wood, Erica M. ;
Murphy, Michael F. .
NEW ENGLAND JOURNAL OF MEDICINE, 2013, 368 (19) :1771-1780
[35]   Impairment of the hemostatic potential of platelets during storage as evaluated by flow cytometry, thrombin generation, and thrombelastography under conditions promoting formation of coated platelets [J].
Svendsen, Mette S. ;
Rojkjaer, Rasmus ;
Kristensen, Annemarie T. ;
Salado-Jimena, Jose A. ;
Kjalke, Marianne ;
Johansson, Par I. .
TRANSFUSION, 2007, 47 (11) :2057-2065
[36]   Platelet Storage Lesion: A New Understanding From a Proteomic Perspective [J].
Thon, Jonathan N. ;
Schubert, Peter ;
Devine, Dana V. .
TRANSFUSION MEDICINE REVIEWS, 2008, 22 (04) :268-290
[37]   Scott syndrome, characterized by impaired transmembrane migration of procoagulant phosphatidylserine and hemorrhagic complications, is an inherited disorder [J].
Toti, F ;
Satta, N ;
Fressinaud, E ;
Meyer, D ;
Freyssinet, JM .
BLOOD, 1996, 87 (04) :1409-1415
[38]   Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads [J].
Tynngard, Nahreen ;
Wallstedt, Maria ;
Sodergren, Anna L. ;
Faxalv, Lars ;
Ramstrom, Sofia .
PLATELETS, 2015, 26 (02) :177-185
[39]   Necrotic platelets provide a procoagulant surface during thrombosis [J].
Vu Minh Hua ;
Abeynaike, Latasha ;
Glaros, Elias ;
Campbell, Heather ;
Pasalic, Leonardo ;
Hogg, Philip J. ;
Chen, Vivien M. Y. .
BLOOD, 2015, 126 (26) :2852-2862
[40]   Therapeutic platelet transfusion versus routine prophylactic transfusion in patients with haematological malignancies: an open-label, multicentre, randomised study [J].
Wandt, Hannes ;
Schaefer-Eckart, Kerstin ;
Wendelin, Knut ;
Pilz, Bettina ;
Wilhelm, Martin ;
Thalheimer, Markus ;
Mahlknecht, Ulrich ;
Ho, Anthony ;
Schaich, Markus ;
Kramer, Michael ;
Kaufmann, Martin ;
Leimer, Lothar ;
Schwerdtfeger, Rainer ;
Conradi, Roland ;
Doelken, Gottfried ;
Klenner, Anne ;
Haenel, Mathias ;
Herbst, Regina ;
Junghanss, Christian ;
Ehninger, Gerhard .
LANCET, 2012, 380 (9850) :1309-1316