Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution

被引：33
|
作者：
Duenas, Maria Emilia [1 ,2 ]
Carlucci, Laura [1 ]
Lee, Young Jin [1 ,2 ]
机构：
[1] US DOE, Ames Lab, Ames, IA 50011 USA
[2] Iowa State Univ Sci & Technol, Dept Chem, Ames, IA 50011 USA
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2016年 / 27卷 / 09期
关键词：
Mass spectrometry imaging; MALDI; Matrix; Recrystallization; Maize; Lipids; High spatial resolution; MASS-SPECTROMETRY; LATENT FINGERMARKS; TOF MS; TISSUE; SUBLIMATION; METABOLITES; EFFICIENCY;
D O I：
10.1007/s13361-016-1422-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 mu m spatial resolution.
引用
收藏
页码：1575 / 1578
页数：4
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