Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications

被引：32

作者：

Ling, Xuefeng B. ^{[1
]}

Park, Jane L. ^{[1
,2
]}

Carroll, Tanya ^{[1
]}

Nguyen, Khoa D. ^{[1
]}

Lau, Kenneth ^{[1
]}

Macaubas, Claudia ^{[1
,2
]}

Chen, Edward ^{[1
]}

Lee, Tzielan ^{[1
]}

Sandborg, Christy ^{[1
,3
]}

Milojevic, Diana

Kanegaye, John T. ^{[4
,6
]}

Gao, Susanna ^{[1
]}

Burns, Jane ^{[5
]}

Schilling, James ^{[1
]}

Mellins, Elizabeth D. ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

[2] Stanford Univ, Program Immunol, Stanford, CA 94305 USA

[3] Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA

[4] Univ Calif San Diego, Sch Med, Dept Emergency Med, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Sch Med, Dept Pediat, La Jolla, CA 92093 USA

[6] Rady Childrens Hosp San Diego, La Jolla, CA USA

来源：

PROTEOMICS | 2010年 / 10卷 / 24期

关键词：

2-D DIGE; Kawasaki disease; MALDI-TOF/TOF MS; Systemic juvenile idiopathic arthritis; Systems biology; ACUTE-PHASE PROTEINS; RHEUMATOID-ARTHRITIS; BACTERIAL-INFECTION; LUPUS-ERYTHEMATOSUS; SYNOVIAL-FLUID; A-IV; DISEASE; ACTIVATION; EXPRESSION; BLOOD;

D O I：

10.1002/pmic.201000298

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Systemic juvenile idiopathic arthritis (SJIA) is a chronic arthritis of children characterized by a combination of arthritis and systemic inflammation. There is usually non-specific laboratory evidence of inflammation at diagnosis but no diagnostic test. Normalized volumes from 89/889 2-D protein spots representing 26 proteins revealed a plasma pattern that distinguishes SJIA flare from quiescence. Highly discriminating spots derived from 15 proteins constitute a robust SJIA flare signature and show specificity for SJIA flare in comparison to active polyarticular juvenile idiopathic arthritis or acute febrile illness. We used 7 available ELISA assays, including one to the complex of S100A8/S100A9, to measure levels of 8 of the15 proteins. Validating our DIGE results, this ELISA panel correctly classified independent SJIA flare samples, and distinguished them from acute febrile illness. Notably, data using the panel suggest its ability to improve on erythrocyte sedimentation rate or C-reactive protein or S100A8/S100A9, either alone or in combination in SJIA F/Q discriminations. Our results also support the panel's potential clinical utility as a predictor of incipient flare (within 9 wk) in SJIA subjects with clinically inactive disease. Pathway analyses of the 15 proteins in the SJIA flare versus quiescence signature corroborate growing evidence for a key role for IL-1 at disease flare.

引用

页码：4415 / 4430

页数：16

共 69 条

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