LncRNA DGCR5 Isoform-1 Silencing Suppresses the Malignant Phenotype of Clear Cell Renal Cell Carcinoma via miR-211-5p/Snail Signal Axis

被引:10
|
作者
Zhong, Guang-Xin [1 ,2 ]
Luo, Dan [1 ,2 ]
Fan, Yi-jun [1 ,2 ]
Wang, Jue [2 ,3 ]
Liu, Bing-Qiang [4 ]
Xu, Zhong-Hua [1 ]
Zhang, Xiang [1 ,2 ]
机构
[1] Shandong Univ, Qilu Hosp, Dept Urol, Jinan, Peoples R China
[2] Shandong Univ, Sch Med, Jinan, Peoples R China
[3] Shandong Univ, Hosp 2, Cent Res Lab, Inst Med Sci, Jinan, Peoples R China
[4] Shandong Univ, Sch Math, Jinan, Peoples R China
来源
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷
基金
中国国家自然科学基金;
关键词
DGCR5; cell proliferation; ceRNA; clear cell renal cell carcinoma; miR-211-5p; LONG NONCODING RNA; DOWN-REGULATION; CANCER; INVASION; PROLIFERATION; PROGRESSION; MIGRATION; METASTASIS; SNAIL;
D O I
10.3389/fcell.2021.700029
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Long non-coding RNAs (lncRNAs) play important roles during the initiation and progression of cancer. We identified DiGeorge Syndrome Critical Region Gene 5 (DGCR5) as a clear cell renal cell carcinoma (ccRCC) cancer- and lineage-specific lncRNA. Agarose gel electrophoresis analysis and sanger sequencing verified two main isoforms of DGCR5 in ccRCC patient tissues and cell lines. Quantitative polymerase chain reaction further demonstrated that the expression level of DGCR5 major isoform (isoform-1) was higher in ccRCC tissues than that in papillary/chromophobe RCC and other multiple solid malignant tumors. We investigate the biological functions of DGCR5 isoform-1 in ccRCC and show that DGCR5 isoform-1 exerts a tumor-promoting effect in ccRCC. DGCR5 isoform-1 is localized in cytoplasm and shares the same binding sequence to the tumor-suppressive miR-211-5p with the epithelial-to-mesenchymal transition key component SNAI. Furthermore, cellular and molecular experiments demonstrate that DGCR5 isoform-1 could sequester miR-211-5p, leading to the elevation of Snail protein and downregulation of its downstream targets and further promoting ccRCC cell proliferation and migration. Thus, our study indicates that DGCR5 isoform-1 could contribute to ccRCC progression by sponging miR-211-5p through regulating the expression of Snail protein and could serve as a reliable diagnostic biomarker in ccRCC.
引用
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页数:12
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