LncRNA DGCR5 Isoform-1 Silencing Suppresses the Malignant Phenotype of Clear Cell Renal Cell Carcinoma via miR-211-5p/Snail Signal Axis

被引:10
|
作者
Zhong, Guang-Xin [1 ,2 ]
Luo, Dan [1 ,2 ]
Fan, Yi-jun [1 ,2 ]
Wang, Jue [2 ,3 ]
Liu, Bing-Qiang [4 ]
Xu, Zhong-Hua [1 ]
Zhang, Xiang [1 ,2 ]
机构
[1] Shandong Univ, Qilu Hosp, Dept Urol, Jinan, Peoples R China
[2] Shandong Univ, Sch Med, Jinan, Peoples R China
[3] Shandong Univ, Hosp 2, Cent Res Lab, Inst Med Sci, Jinan, Peoples R China
[4] Shandong Univ, Sch Math, Jinan, Peoples R China
来源
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷
基金
中国国家自然科学基金;
关键词
DGCR5; cell proliferation; ceRNA; clear cell renal cell carcinoma; miR-211-5p; LONG NONCODING RNA; DOWN-REGULATION; CANCER; INVASION; PROLIFERATION; PROGRESSION; MIGRATION; METASTASIS; SNAIL;
D O I
10.3389/fcell.2021.700029
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Long non-coding RNAs (lncRNAs) play important roles during the initiation and progression of cancer. We identified DiGeorge Syndrome Critical Region Gene 5 (DGCR5) as a clear cell renal cell carcinoma (ccRCC) cancer- and lineage-specific lncRNA. Agarose gel electrophoresis analysis and sanger sequencing verified two main isoforms of DGCR5 in ccRCC patient tissues and cell lines. Quantitative polymerase chain reaction further demonstrated that the expression level of DGCR5 major isoform (isoform-1) was higher in ccRCC tissues than that in papillary/chromophobe RCC and other multiple solid malignant tumors. We investigate the biological functions of DGCR5 isoform-1 in ccRCC and show that DGCR5 isoform-1 exerts a tumor-promoting effect in ccRCC. DGCR5 isoform-1 is localized in cytoplasm and shares the same binding sequence to the tumor-suppressive miR-211-5p with the epithelial-to-mesenchymal transition key component SNAI. Furthermore, cellular and molecular experiments demonstrate that DGCR5 isoform-1 could sequester miR-211-5p, leading to the elevation of Snail protein and downregulation of its downstream targets and further promoting ccRCC cell proliferation and migration. Thus, our study indicates that DGCR5 isoform-1 could contribute to ccRCC progression by sponging miR-211-5p through regulating the expression of Snail protein and could serve as a reliable diagnostic biomarker in ccRCC.
引用
收藏
页数:12
相关论文
共 50 条
  • [21] LncRNA GAPLINC Promotes Renal Cell Cancer Tumorigenesis by Targeting the miR-135b-5p/CSF1 Axis
    Wang, Siyuan
    Yang, Xiaorong
    Xie, Wenjie
    Fu, Shengqiang
    Chen, Qiang
    Li, Zhilong
    Zhang, Zhicheng
    Sun, Ting
    Gong, Binbin
    Ma, Ming
    FRONTIERS IN ONCOLOGY, 2021, 11
  • [22] LncRNA NORAD promotes proliferation, migration and angiogenesis of hepatocellular carcinoma cells through targeting miR-211-5p/FOXD1/VEGF-A axis
    Sun, Dong-Sheng
    Guan, Cang-Hai
    Wang, Wei-Na
    Hu, Zeng-Tao
    Zhao, Yu-Qiao
    Jiang, Xing-Ming
    MICROVASCULAR RESEARCH, 2021, 134
  • [23] LncRNA PART1 Exerts Tumor-Suppressive Functions in Tongue Squamous Cell Carcinoma via miR-503-5p
    Zhao, Xiqun
    Hong, Yanqing
    Cheng, Qingyuan
    Guo, Lixin
    ONCOTARGETS AND THERAPY, 2020, 13 : 9977 - 9989
  • [24] LncRNA GIHCG promoted the proliferation and migration of renal cell carcinoma through regulating miR-499a-5p/XIAP axis
    Zhu, Shu-Ying
    Zou, Hong-Chang
    Gao, Ming-Ming
    Chen, Yan-Xia
    Xu, Man
    Qin, Xiao-Hua
    TRANSLATIONAL ONCOLOGY, 2022, 20
  • [25] Long Non-Coding RNA TUG1 Promotes Cell Proliferation and Inhibits Cell Apoptosis, Autophagy in Clear Cell Renal Cell Carcinoma via MiR-31-5p/ FLOT1 Axis
    Lv, Dong
    Xiang, Ying
    Yang, Qi
    Yao, Juncheng
    Dong, Qiang
    ONCOTARGETS AND THERAPY, 2020, 13 : 5857 - 5868
  • [26] LINC00511 knockdown suppresses glioma cell malignant progression through miR-15a-5p/AEBP1 axis
    Liu, Zhen
    Tao, Bei
    Li, Linkun
    Liu, Pin
    Xia, Kaiguo
    Zhong, Chuanhong
    BRAIN RESEARCH BULLETIN, 2021, 173 : 82 - 96
  • [27] MiR-212-5p inhibits the malignant behavior of clear cell renal cell carcinoma cells by targeting TBX15
    Deng, J. -H.
    Zheng, G. -Y.
    Li, H. -Z.
    Ji, Z. -G.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2019, 23 (24) : 10699 - 10707
  • [28] lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis
    Zeng, Jiawei
    Li, Yuanmeng
    Wang, Yaodong
    Xie, Gang
    Feng, Qian
    Yang, Yuwei
    Feng, Jiafu
    OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2020, 2020 (2020)
  • [29] Silencing of circular RNA PUM1 inhibits clear cell renal cell carcinoma progression through the miR-340-5p/FABP7 axis
    Zeng, Fanchang
    Luo, Liumei
    Song, Mi
    Li, Daoyuan
    JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION, 2022, 42 (02) : 141 - 150
  • [30] Comprehensive analysis reveals the involvement of hsa_circ_0037858/miR-5000-3p/FMR1 axis in malignant metastasis of clear cell renal cell carcinoma
    Yuan, Nianyong
    Li, Guowei
    AGING-US, 2023, 15 (12): : 5399 - 5411