A mecA-Negative Strain of Methicillin-Resistant Staphylococcus aureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes

被引：85
|
作者：
Banerjee, Ritu [1 ]
Gretes, Michael [2 ]
Harlem, Christopher [3 ]
Basuino, Li [3 ]
Chambers, Henry F. [3 ]
机构：
[1] Mayo Clin, Div Pediat Infect Dis, Rochester, MN 55905 USA
[2] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC, Canada
[3] San Francisco Gen Hosp, Div Infect Dis, San Francisco, CA 94110 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 11期
关键词：
PENICILLIN-BINDING PROTEINS; STREPTOCOCCUS-PNEUMONIAE; VANCOMYCIN RESISTANCE; ESCHERICHIA-COLI; CEFTOBIPROLE; DOMAIN; PBP4; SUSCEPTIBILITY; EXPRESSION; ACRB;
D O I：
10.1128/AAC.00594-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of beta-lactams but was hypersusceptible to cefoxitin.
引用
收藏
页码：4900 / 4902
页数：3
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