A mecA-Negative Strain of Methicillin-Resistant Staphylococcus aureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes

被引：85
|
作者：
Banerjee, Ritu [1 ]
Gretes, Michael [2 ]
Harlem, Christopher [3 ]
Basuino, Li [3 ]
Chambers, Henry F. [3 ]
机构：
[1] Mayo Clin, Div Pediat Infect Dis, Rochester, MN 55905 USA
[2] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC, Canada
[3] San Francisco Gen Hosp, Div Infect Dis, San Francisco, CA 94110 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 11期
关键词：
PENICILLIN-BINDING PROTEINS; STREPTOCOCCUS-PNEUMONIAE; VANCOMYCIN RESISTANCE; ESCHERICHIA-COLI; CEFTOBIPROLE; DOMAIN; PBP4; SUSCEPTIBILITY; EXPRESSION; ACRB;
D O I：
10.1128/AAC.00594-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of beta-lactams but was hypersusceptible to cefoxitin.
引用
收藏
页码：4900 / 4902
页数：3
相关论文
共 49 条
  • [21] SpoVG Regulates Cell Wall Metabolism and Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus Strain N315
    Liu, Xiaoyu
    Zhang, Shijie
    Sun, Baolin
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (06) : 3455 - 3461
  • [22] High-level mupirocin resistance in methicillin-resistant staphylococci isolated from dogs and cats
    Kizerwetter-Swida, Magdalena
    Chrobak-Chmiel, Dorota
    Rzewuska, Magdalena
    BMC VETERINARY RESEARCH, 2019, 15 (1)
  • [23] Mutation of RNA Polymerase β-Subunit Gene Promotes Heterogeneous-to-Homogeneous Conversion of β-Lactam Resistance in Methicillin-Resistant Staphylococcus aureus
    Aiba, Yoshifumi
    Katayama, Yuki
    Hishinuma, Tomomi
    Murakami-Kuroda, Hiroko
    Cui, Longzhu
    Hiramatsu, Keiichi
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (10) : 4861 - 4871
  • [24] Frequency of Aminoglycoside-Resistance Genes in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitalized Patients
    Mahdiyoun, Seyed Mohsen
    Kazemian, Hossein
    Ahanjan, Mohammad
    Houri, Hamidreza
    Goudarzi, Mehdi
    JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2016, 9 (08)
  • [25] In vivo efficacy of β-lactam/tripropeptin C in a mouse septicemia model and the mechanism of reverse β-lactam resistance in methicillin-resistant Staphylococcus aureus mediated by tripropeptin C
    Hashizume, Hideki
    Takahashi, Yoshiaki
    Masuda, Tohru
    Ohba, Shun-ichi
    Ohishi, Tomokazu
    Kawada, Manabu
    Igarashi, Masayuki
    JOURNAL OF ANTIBIOTICS, 2018, 71 (01) : 79 - 85
  • [26] Study of SarA by DNA Affinity Capture Assay (DACA) Employing Three Promoters of Key Virulence and Resistance Genes in Methicillin-Resistant Staphylococcus aureus
    Kim, Byungchan
    Lee, Hong-Ju
    Jo, Sung-Hyun
    Kim, Min-Gyu
    Lee, Yeonhee
    Lee, Wonsik
    Kim, Wooseong
    Joo, Hwang-Soo
    Kim, Yun-Gon
    Kim, Jae-Seok
    Yang, Yung-Hun
    ANTIBIOTICS-BASEL, 2022, 11 (12):
  • [27] Characterization of antibiotic resistance and virulence genes of ocular methicillin-resistant Staphylococcus aureus strains through complete genome analysis
    Kathirvel, Kandasamy
    Rudhra, O.
    Rajapandian, Siva Ganesa Karthikeyan
    Prajna, Namperumalsamy Venkatesh
    Lalitha, Prajna
    Devarajan, Bharanidharan
    EXPERIMENTAL EYE RESEARCH, 2021, 212
  • [28] Basis of Virulence in a Panton-Valentine Leukocidin-Negative Community-Associated Methicillin-Resistant Staphylococcus aureus Strain
    Chen, Yan
    Yeh, Anthony J.
    Cheung, Gordon Y. C.
    Villaruz, Amer E.
    Tan, Vee Y.
    Joo, Hwang-Soo
    Chatterjee, Som S.
    Yu, Yunsong
    Otto, Michael
    JOURNAL OF INFECTIOUS DISEASES, 2015, 211 (03) : 472 - 480
  • [29] In-Host Evolution of Daptomycin Resistance and Heteroresistance in Methicillin-Resistant Staphylococcus aureus Strains From Three Endocarditis Patients
    Ji, Shujuan
    Jiang, Shengnan
    Wei, Xiang
    Sun, Lu
    Wang, Haiping
    Zhao, Feng
    Chen, Yan
    Yu, Yunsong
    JOURNAL OF INFECTIOUS DISEASES, 2020, 221 : S243 - S252
  • [30] Investigation of Mutations and Expression Level of GraSR and WalKR Systems Associated with Vancomycin Non-Susceptibility in Methicillin-Resistant Staphylococcus aureus
    Aghaei, Mahsa
    Asadpour, Leila
    Arasteh, Amir
    MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, 2024, 39 (01) : 65 - 73
12345