Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima

被引：5
|
作者：
Ihsanawati [1 ]
Kumasaka, T [1 ]
Kaneko, T [1 ]
Morokuma, C [1 ]
Nakamura, S [1 ]
Tanaka, N [1 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, Kanagawa 2268501, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷
关键词：
D O I：
10.1107/S0907444903015397
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Xylanases catalyze the hydrolysis of the beta-1,4-glycosidic bonds of xylan, which is the second most abundant component of plant cell walls after cellulose. The recombinant xylanase 10B from Thermotoga maritima MSB8 was prepared and crystallized by the sitting-drop vapour-diffusion method using 40 mM zinc acetate, 20 mM MES buffer pH 6.0 and 3% ethanol. Intensity data were collected to 2.5 Angstrom resolution at beamline BL26B2 of SPring-8. Preliminary X-ray analysis showed that the crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 77.3, b = 80.6, c = 58.2 Angstrom and one molecule per asymmetric unit.
引用
收藏
页码：1659 / 1661
页数：3
相关论文
共 50 条
  • [11] Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium
    Nishimoto, M
    Fushinobu, S
    Miyanaga, A
    Wakagi, T
    Shoun, H
    Sakka, K
    Ohmiya, K
    Nirasawa, S
    Kitaoka, M
    Hayashi, K
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 342 - 343
  • [12] Crystallization and preliminary X-ray crystallographic analysis of CheW from Thermotoga maritima: a coupling protein of CheA and the chemotaxis receptor
    Park, SangYoun
    Crane, Brian R.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 504 - 506
  • [13] Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima
    Cicmil, Nenad
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 193 - 195
  • [14] Crystallization and preliminary X-ray structure analysis of isocitrate dehydrogenase from two hyperthermophiles, Aeropyrum pernix and Thermotoga maritima
    Karlström, M
    Steen, IH
    Tibbelin, G
    Lien, T
    Birkeland, NK
    Ladenstein, R
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 2162 - 2164
  • [15] Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase from Thermotoga neapolitana strain DSM 4359
    Okazaki, Fumiyoshi
    Ogino, Chiaki
    Kondo, Akihiko
    Mikami, Bunzo
    Kurebayashi, Yoichi
    Tsuruta, Hiroki
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 779 - 781
  • [16] Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8
    Ihsanawati
    Kumasaka, T
    Kaneko, T
    Morokuma, C
    Yatsunami, R
    Sato, T
    Nakamura, S
    Tanaka, N
    PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2005, 61 (04) : 999 - 1009
  • [17] Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima
    Barbey, Carole
    Rouhier, Nicolas
    Haouz, Ahmed
    Navaza, Alda
    Jacquot, Jean-Pierre
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 29 - 31
  • [18] Crystallization and preliminary X-ray crystallographic studies of XynX, a family 10 xylanase from Aeromonas punctata ME-1
    Fujimoto, Z
    Usui, K
    Kondo, Y
    Yasui, K
    Kawai, K
    Suzuki, T
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 255 - 257
  • [19] Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima
    Ethayathulla, Abdul S.
    Bessho, Yoshitaka
    Shinkai, Akeo
    Padmanabhan, Balasundaram
    Singh, Tej P.
    Kaur, Punit
    Yokoyama, Shigeyuki
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 498 - 500
  • [20] Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis
    Van Petegem, F
    Collins, T
    Meuwis, MA
    Gerday, C
    Feller, G
    Van Beeumen, J
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 1494 - 1496
12345