Nucleotide sequence and characterization of a novel cefotaxime-hydrolyzing β-lactamase (CTX-M-10) isolated in Spain

被引：83
作者：
Oliver, A [1 ]
Pérez-Díaz, JC [1 ]
Coque, TM [1 ]
Baquero, F [1 ]
Cantón, R [1 ]
机构：
[1] Hosp Ramon & Cajal, Microbiol Serv, E-28034 Madrid, Spain
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 02期
关键词：
D O I：
10.1128/AAC.45.2.616-620.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A cefotaxime-resistant, ceftazidime-susceptible Escherichia coli isolate was obtained from a patient with sepsis in 1997, from which a beta -lactamase with a pI of 8.2 was cloned. Cephaloridine and cefotaxime relative hydrolysis rates were 167 and 81, respectively (penicillin G rate = 100), whereas ceftazidime hydrolysis was not detected. The nucleotide sequence revealed a bla gene related to that coding for CTX-M-3. Despite 21 nucleotide substitutions, only 2 determined amino acid changes (Ala27Val and Arg38Gln). The amino acid sequence identify between this enzyme, designated CTX-M-10 and the chromosomal beta -lactamase of Kluyvera. ascorbata was 81%.
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页码：616 / 620
页数：5
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