Effect of carbon sources on lipid accumulation in Rhodococcus cells

被引：35

作者：

Cortes, Maria Ana L. R. M. ^{[1
]}

de Carvalho, Carla C. C. R. ^{[1
]}

机构：

[1] Univ Lisbon, Inst Super Tecn, Dept Bioengn, Inst Biotechnol & Bioengn,Ctr Biol & Chem Engn, P-1049001 Lisbon, Portugal

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2015年 / 94卷

关键词：

Microbial growth; Biosynthesis; Fermentation; Biofuel; Specialized lipid; Triacylglycerol; BIODIESEL PRODUCTION; ERYTHROPOLIS CELLS; STORAGE LIPIDS; OPACUS PD630; FATTY-ACIDS; TRIACYLGLYCEROLS; ADAPTATION; MICROALGAE; GROWTH; FUEL;

D O I：

10.1016/j.bej.2014.11.017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

New regenerative and ecologically friendly processes to produce energy are required to replace petroleum-based derived combustibles. The objective of this work was to assess the usefulness Of Rhodococcus erythropolis DCL14 and Rhodococcus opacus PWD4 cells to produce lipids for biodiesel blends. These cells accumulated triacylglycerols (TAGs), the most relevant lipids for biodiesel production, in addition to other lipids, including apolar lipids and phospholipids, which contained fatty acids that could be esterified. The proportion between phospholipids and storage lipids was dependent on carbon source and culture age. The estimated cetane number of the fatty acid blends produced ranged between 60.5 and 61.7 for R. erythropolis and 50.5 and 69.8 for R. opacus. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：100 / 105

页数：6

共 41 条

[11] GROWTH ON OCTANE ALTERS THE MEMBRANE LIPID FATTY-ACIDS OF PSEUDOMONAS-OLEOVORANS DUE TO THE INDUCTION OF ALKB AND SYNTHESIS OF OCTANOL [J].

CHEN, Q ;

JANSSEN, DB ;

WITHOLT, B .

JOURNAL OF BACTERIOLOGY, 1995, 177 (23) :6894-6901

[12] Biodiesel from microalgae [J].

Chisti, Yusuf .

BIOTECHNOLOGY ADVANCES, 2007, 25 (03) :294-306

[13] CHROMATOGRAPHIC ANALYSIS OF PHOSPHOLIPIDS [J].

CHRISTIE, WW .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1985, 181 (03) :171-182

[14]

Christie WW., 2010, Lipid analysis : isolation, separation, identification and lipidomic analysis, V4th

[15] Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture [J].

Daniel, J ;

Deb, C ;

Dubey, VS ;

Sirakova, TD ;

Abomoelak, B ;

Morbidoni, HR ;

Kolattukudy, PE .

JOURNAL OF BACTERIOLOGY, 2004, 186 (15) :5017-5030

[16] Mycobacterium tuberculosis Uses Host Triacylglycerol to Accumulate Lipid Droplets and Acquires a Dormancy-Like Phenotype in Lipid-Loaded Macrophages [J].

Daniel, Jaiyanth ;

Maamar, Hedia ;

Deb, Chirajyoti ;

Sirakova, Tatiana D. ;

Kolattukudy, Pappachan E. .

PLOS PATHOGENS, 2011, 7 (06)

[17] Degradation of hydrocarbons and alcohols by Rhodococcus erythropolis DCL14:: A comparison in scale performance [J].

De Carvalho, Carla C. C. R. ;

Marques, Marco P. C. ;

Fernandes, Pedro ;

Da Fonseca, M. Manuela R. .

BIOCATALYSIS AND BIOTRANSFORMATION, 2007, 25 (2-4) :144-150

[18] Adaptation of Rhodococcus erythropolis cells to high concentrations of toluene [J].

de Carvalho, Carla C. C. R. ;

Fatal, Vanessa ;

Alves, Sebastiao S. ;

da Fonseca, M. Manuela R. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 76 (06) :1423-1430

[19] Rapid adaptation of Rhodococcus erythropolis cells to salt stress by synthesizing polyunsaturated fatty acids [J].

de Carvalho, Carla C. C. R. ;

Marques, Marco P. C. ;

Hachicho, Nancy ;

Heipieper, Hermann J. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (12) :5599-5606

[20] Adaptation of Rhodococcus erythropolis cells for growth and bioremediation under extreme conditions [J].

de Carvalho, Carla C. C. R. .

RESEARCH IN MICROBIOLOGY, 2012, 163 (02) :125-136

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