Effects of N-acetylcysteine treatment on oxidative stress in acetic acid-induced experimental colitis in rats

被引:58
作者
Akgun, E [1 ]
Çaliskan, C
Celik, HA
Ozutemiz, AO
Tuncyurek, M
Aydin, HH
机构
[1] Ege Univ, Sch Med, Dept Gen Surg, TR-35100 Izmir, Turkey
[2] Ege Univ, Sch Med, Dept Biochem, TR-35100 Izmir, Turkey
[3] Ege Univ, Sch Med, Dept Gastroenterol, TR-35100 Izmir, Turkey
[4] Ege Univ, Sch Med, Dept Pathol, TR-35100 Izmir, Turkey
关键词
N-acetylcysteine; experimental colitis; oxidative stress; antioxidant; rat;
D O I
10.1177/147323000503300207
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We assessed the possible protective effects of N-acetylcysteine (NAC) against toxic damage in the rat colon. Two doses of NAC (20 mg/kg and 100 mg/kg) given for 2 days and 7 days after acetic acid administration (to induce colitis) were tested. NAC was dissolved in saline and administered locally (intracolonic), systemically (intraperitoneal) or in a combination (intracolonic and intraperitoneal). Several parameters, including macroscopic and histopathological scores and myeloperoxidase, glutathione and nitric oxide concentrations were measured using standard assay procedures. Treatment with 100 mg/kg NAC for 7 days significantly decreased tissue myeloperoxidase, glutathione and nitric oxide concentrations. The 20 mg/kg dose had no protective effects. The data indicate that NAC substantially reduced the degree of colonic injury, probably by regulating free radical production and inhibiting inflammation. It may, therefore, have a role in the treatment of inflammatory bowel disease.
引用
收藏
页码:196 / 206
页数:11
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