Triazophos up-regulated gene expression in the female brown planthopper, Nilaparvata lugens

被引：49
作者：
Bao, Yan-Yuan
Li, Bao-Ling
Liu, Zhao-Bu
Xue, Jian
Zhu, Zeng-Rong
Cheng, Jia-An
Zhang, Chuan-Xi [1 ]
机构：
[1] Zhejiang Univ, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China
来源：
JOURNAL OF INSECT PHYSIOLOGY | 2010年 / 56卷 / 09期
关键词：
Nilaparvata lugens; Triazophos; Resurgence; Suppression subtractive hybridization; PURINE NUCLEOSIDE PHOSPHORYLASE; REDUCTASE GENE; INSECTICIDE; RESISTANCE; BYSTIN; OVEREXPRESSION; POPULATION; RICE;
D O I：
10.1016/j.jinsphys.2010.03.004
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
The widespread use of insecticides has caused the resurgence of the brown planthopper, Nilaparvata lugens, in Asia. In this study, we investigated an organo-phosphorous insecticide, triazophos, and its ability to induce gene expression variation in female N. lugens nymphs just before emergence. By using the suppression subtractive hybridization method, a triazophos-induced cDNA library was constructed. In total, 402 differentially expressed cDNA clones were obtained. Real-time qPCR analysis confirmed that triazophos up-regulated the expression of six candidate genes at the transcript level in nymphs on day 3 of the 5th instar. These genes encode N. lugens vitellogenin, bystin, multidrug resistance protein (MRP), purine nucleoside phosphorylase (PNP), pyrroline-5-carboxylate reductase (P5CR) and carboxylesterase. Our results imply that the up-regulation of these genes may be involved in the induction of N. lugens female reproduction or resistance to insecticides. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1087 / 1094
页数：8
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