Identification of SLEEPLESS, a sleep-promoting factor

被引：231

作者：

Koh, Kyunghee ^{[1
]}

Joiner, William J. ^{[1
]}

Wu, Mark N. ^{[2
]}

Yue, Zhifeng ^{[1
]}

Smith, Corinne J. ^{[1
]}

Sehgal, Amita ^{[1
]}

机构：

[1] Univ Penn, Howard Hughes Med Inst, Dept Neurosci, Philadelphia, PA 19104 USA

[2] Univ Penn, Div Sleep Med, Dept Neurol, Philadelphia, PA 19104 USA

来源：

SCIENCE | 2008年 / 321卷 / 5887期

关键词：

D O I：

10.1126/science.1155942

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sleep is an essential process conserved from flies to humans. The importance of sleep is underscored by its tight homeostatic control. Through a forward genetic screen, we identified a gene, sleepless, required for sleep in Drosophila. The sleepless gene encodes a brain-enriched, glycosylphosphatidylinositol-anchored protein. Loss of SLEEPLESS protein caused an extreme (> 80%) reduction in sleep; a moderate reduction in SLEEPLESS had minimal effects on baseline sleep but markedly reduced the amount of recovery sleep after sleep deprivation. Genetic and molecular analyses revealed that quiver, a mutation that impairs Shaker-dependent potassium current, is an allele of sleepless. Consistent with this finding, Shaker protein levels were reduced in sleepless mutants. We propose that SLEEPLESS is a signaling molecule that connects sleep drive to lowered membrane excitability.

引用

页码：372 / 376

页数：5

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