Molecular detection and identification of Rickettsiales pathogens in dog ticks from Costa Rica

被引：30

作者：

Campos-Calderon, Liliana ^{[1
]}

Abrego-Sanchez, Leyda ^{[2
]}

Solorzano-Morales, Antony ^{[1
]}

Alberti, Alberto ^{[3
]}

Tore, Gessica ^{[3
]}

Zobba, Rosanna ^{[3
]}

Jimenez-Rocha, Ana E. ^{[1
]}

Dolz, Gaby ^{[1
,2
]}

机构：

[1] Univ Nacl, Escuela Med Vet, Campus Benjamin Nunez, Barreal De Heredia, Costa Rica

[2] Univ Nacl, Enfermedades Trop, Posgrad Reg Ciencias Vet Trop, Campus Benjamin Nunez, Barreal De Heredia, Costa Rica

[3] Univ Sassari, Dept Vet Med, Via Vienna 2, I-07100 Sassari, Italy

来源：

TICKS AND TICK-BORNE DISEASES | 2016年 / 7卷 / 06期

关键词：

Rhipicephalus sanguineus s.l; SANGUINEUS GROUP TICKS; RHIPICEPHALUS-SANGUINEUS; ANAPLASMA-PLATYS; EHRLICHIA-PLATYS; BORNE PATHOGENS; SPOTTED-FEVER; ACARI; IXODIDAE; STRAINS; VECTOR;

D O I：

10.1016/j.ttbdis.2016.07.015

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in Central America. This study focuses on the detection and molecular identification of species belonging to selected bacterial genera (Ehrlichia, Anaplasma and Rickettsia) in ticks sampled from dogs in Costa Rica by targeting several genes: 16S rRNA/dsb genes for Ehrlichia; 16S rRNAlgroEL genes for Anaplasma, and ompA/gItA/groEL genes for Rickettsia. PCR and sequence analyses provides evidences of Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum infection in Rhipicephalus sanguineus s.l ticks, and allow establishing the presence of Rickettsia monacensis in Ixodes boliviensis. Furthermore, the presence of recently discovered Mediterranean A. platys-like strains is reported for the first time in Central America. Results provide new background on geographical distribution of selected tick-transmitted bacterial pathogens in Costa Rica and on their molecular epidemiology, and are pivotal to the development of effective and reliable diagnostic tools in Central America. (C) 2016 Elsevier GmbH. All rights reserved.

引用

页码：1198 / 1202

页数：5

共 44 条

[1]

Abrego V., 2009, CIENCIAS VET COSTA R, V27, P71

[2] Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States [J].

Alberti, A ;

Zobba, R ;

Chessa, B ;

Addis, MF ;

Sparagano, O ;

Parpaglia, MLP ;

Cubeddu, T ;

Pintori, G ;

Pittau, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) :6418-6422

[3]

Alvarez Calderon Victor, 2005, Brenesia, V63-64, P81

[4]

Alvarez V., 2006, Ciencias Veterinarias (Heredia), V24, P9

[5]

Barrantes-Gonzalez A., 2013, ACTA MED COSTARRIC, V55, P68

[6]

Bechara G. H., 2006, CARRAPATOS IMPORTANC

[7] Characterization of spotted fever group rickettsiae in flea and tick specimens from northern Peru [J].

Blair, PJ ;

Jiang, J ;

Schoeler, GB ;

Moron, C ;

Anaya, E ;

Cespedes, M ;

Cruz, C ;

Felices, V ;

Guevara, C ;

Mendoza, L ;

Villaseca, P ;

Sumner, JW ;

Richards, AL ;

Olson, JG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :4961-4967

[8] Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii [J].

Breitschwerdt, EB ;

Hegarty, BC ;

Hancock, SI .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2645-2651

[9] Canine Granulocytic Anaplasmosis: A Review [J].

Carrade, D. D. ;

Foley, J. E. ;

Borjesson, D. L. ;

Sykes, J. E. .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 2009, 23 (06) :1129-1141

[10] Molecular detection and groEL typing of Rickettsia aeschlimannii in Sardinian ticks [J].

Chisu, Valentina ;

Zobba, Rosanna ;

Foxi, Cipriano ;

Pisu, Danilo ;

Masala, Giovanna ;

Alberti, Alberto .

PARASITOLOGY RESEARCH, 2016, 115 (09) :3323-3328

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