Extraction of glutathione s-transferase from bovine liver

被引：1

作者：

Lopes Torres, Maria Celia ^{[1
]}

Ferreira Soares, Nilda de Fatima ^{[2
]}

Marques Pereira, Jose Antnio ^{[2
]}

机构：

[1] Univ Fed Goias, Escola Agron & Engn Alimentos, BR-74001970 Goiania, Go, Brazil

[2] Univ Fed Vicosa, Dept Tecnol Alimentos, BR-36570000 Vicosa, MG, Brazil

来源：

CIENCIA E AGROTECNOLOGIA | 2006年 / 30卷 / 02期

关键词：

Glutathione S-transferase; extraction; bovine liver; enzyme activity;

D O I：

10.1590/S1413-70542006000200016

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Considering the detoxication functions of Glutathione S-transferase (GST) enzyme, that is important against oxidative stress, cancer and others degenerative diseases, this study aimed to evaluate the stability and activity of Glutathione S-transferase extracted from bovine liver, which is commercially available at low cost. The extraction was done in four steps (homogenization/centrifugation, passage through column containing diethylaminoethyl-cellulose (DEAE), precipitation with ammonium sulfate and passing through column of carboxy-methyl-cellulose (CMC). The extract thus obtained showed activity with 1 chloro 2, 4 dinitrobenzene, in the presence of reduced glutation. The specific activity of the final extract was 5 times greater than the crude centrifuged extract, and was stable for 70 days when stored at 5 degrees C.

引用

页码：302 / 307

页数：6

共 14 条

[1] Reengineering the glutathione S-transferase scaffold:: A rational design strategy pays off [J].

Babbitt, PC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (19) :10298-10300

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] Diverse expression profiles of glutathione-S-transferase subunits in mammalian urinary bladders [J].

Chico, DE ;

Listowsky, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 435 (01) :56-64

[4] Hazard characterisation of chemicals in food and diet: dose response, mechanisms and extrapolation issues [J].

Dybing, E ;

Doe, J ;

Groten, J ;

Kleiner, J ;

O'Brien, J ;

Renwick, AG ;

Schlatter, J ;

Steinberg, P ;

Tritscher, A ;

Walker, R ;

Younes, M .

FOOD AND CHEMICAL TOXICOLOGY, 2002, 40 (2-3) :237-282

[5]

Habig W H, 1981, Methods Enzymol, V77, P218

[6]

HABIG WH, 1974, J BIOL CHEM, V249, P7130

[7] Glutathione transferases [J].

Hayes, JD ;

Flanagan, JU ;

Jowsey, IR .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2005, 45 :51-88

[8] The glutathione S-Transferase supergene family: Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance [J].

Hayes, JD ;

Pulford, DJ .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 30 (06) :445-600

[9] Glutathione turnover is increased during the acute phase of sepsis in rats [J].

Malmezat, T ;

Breuillé, D ;

Capitan, P ;

Mirand, PP ;

Obled, C .

JOURNAL OF NUTRITION, 2000, 130 (05) :1239-1246

[10]

OGA S, 1996, FUNDAMENTOS TOXICOLO, P9

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