Structure insights into the molecular mechanism of the interaction between UHRF2 and PCNA

被引：9

作者：

Chen, Wanbiao ^{[1
,2
]}

Wu, Minhao ^{[1
,2
]}

Hang, Tianrong ^{[1
,2
]}

Wang, Chengliang ^{[1
,2
]}

Zhang, Xuan ^{[1
,2
]}

Zang, Jianye ^{[1
,2
]}

机构：

[1] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Collaborat Innovat Ctr Chem Life Sci,CAS Ctr Exce, 96 Jinzhai Rd, Hefei 230026, Anhui, Peoples R China

[2] Chinese Acad Sci, Key Lab Struct Biol, Hefei 230026, Anhui, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 494卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

UHRF2; PIP-box; PCNA; Structure; Molecular mechanism; CELL-CYCLE NETWORK; CRYSTAL-STRUCTURE; UBIQUITIN LIGASE; NIRF; BINDING;

D O I：

10.1016/j.bbrc.2017.09.102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UHRF2 (Ubiquitin-like with PHD and ring finger domains 2) is an E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications and cell cycle regulation by interacting with multiple epigenetic or cell-cycle related proteins. Previous studied have identified PCNA (Proliferating cell nuclear antigen) as an interacting partner of UHRF2 by using the antibody microarray. However, the molecular mechanism and the function of UHRF2-PCNA interaction remains unclear. Here, we report the complex structure of PCNA and the peptide ((784)NEILQTLLDLFFPGYSK(800)) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：575 / 580

页数：6

共 22 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution
Adams, Paul D.
Afonine, Pavel V.
Bunkoczi, Gabor
Chen, Vincent B.
Davis, Ian W.
Echols, Nathaniel
Headd, Jeffrey J.
Hung, Li-Wei
Kapral, Gary J.
Grosse-Kunstleve, Ralf W.
McCoy, Airlie J.
Moriarty, Nigel W.
Oeffner, Robert
Read, Randy J.
Richardson, David C.
Richardson, Jane S.
Terwilliger, Thomas C.
Zwart, Peter H.
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 213 - 221
[2] [Anonymous], ENCY OF AIDS, DOI [DOI 10.1007/978-1-4614-9610-6_250-1, DOI 10.1007/978-1-4939-7101-5_205]
[3] MolProbity: all-atom structure validation for macromolecular crystallography
Chen, Vincent B.
Arendall, W. Bryan, III
Headd, Jeffrey J.
Keedy, Daniel A.
Immormino, Robert M.
Kapral, Gary J.
Murray, Laura W.
Richardson, Jane S.
Richardson, David C.
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 12 - 21
[4] Coot:: model-building tools for molecular graphics
Emsley, P
Cowtan, K
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 2126 - 2132
[5] Structure of a sliding clamp on DNA
Georgescu, Roxana E.
Kim, Seung-Sup
Yurieva, Olga
Kuriyan, John
Kong, Xiang-Peng
O'Donnell, Mike
[J]. CELL, 2008, 132 (01) : 43 - 54
[6] TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress
Hoffmann, Saskia
Smedegaard, Stine
Nakamura, Kyosuke
Mortuza, Gulnahar B.
Raschle, Markus
Ibanez de Opakua, Alain
Oka, Yasuyoshi
Feng, Yunpeng
Blanco, Francisco J.
Mann, Matthias
Montoya, Guillermo
Groth, Anja
Bekker-Jensen, Simon
Mailand, Niels
[J]. JOURNAL OF CELL BIOLOGY, 2016, 212 (01) : 63 - 75
[7] CRYSTAL-STRUCTURE OF THE EUKARYOTIC DNA-POLYMERASE PROCESSIVITY FACTOR PCNA
KRISHNA, TSR
KONG, XP
GARY, S
BURGERS, PM
KURIYAN, J
[J]. CELL, 1994, 79 (07) : 1233 - 1243
[8] NIRF induces G1 arrest and associates with Cdk2
Li, YY
Mori, T
Hata, H
Homma, Y
Kochi, H
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 319 (02) : 464 - 468
[9] Uhrf2 is important for DNA damage response in vascular smooth muscle cells
Luo, Tao
Cui, Shijun
Bian, Chunjing
Yu, Xiaochun
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 441 (01) : 65 - 70
[10] Matsumoto Y, 2001, PROG NUCLEIC ACID RE, V68, P129

← 1 2 3 →