The steady-state kinetics of hydrogen peroxide cooxidation of ascorbic acid and potassium ferrocyanide catalyzed by horseradish peroxidase

被引：0

作者：

Rogozhin, VV ^{[1
]}

Verkhoturov, VV ^{[1
]}

机构：

[1] Yakutsk State Agr Acad, Yakutsk 677018, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 1999年 / 25卷 / 01期

关键词：

horseradish peroxidase; ascorbic acid; potassium ferrocyanide;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The steady-state kinetics of hydrogen peroxide cooxidation of ascorbic acid and potassium ferrocyanide catalyzed by horseradish peroxidase was studied. The values of k(cat) and K-m were determined at pH 5-7. In the cooxidation of ascorbic acid and potassium ferrocyanide, the peroxidase oxidation of ferrocyanide is inhibited by ascorbic acid, whereas ferrocyanide itself activates the oxidation of ascorbic acid. The kinetic scheme of the reaction was suggested.

引用

页码：70 / 73

页数：4

共 17 条

[1]

Albreacht HO, 1928, Z PHYS CHEM-STOCH VE, V136, P321

[2]

[Anonymous], BIOCHIMIYA

[3]

Berezin I.V., 1976, PRAKTICHESKII KURS K

[4] THE PROPERTIES OF THE ENZYME-SUBSTRATE COMPOUNDS OF HORSE-RADISH AND LACTO-PEROXIDASE [J].