hnRNPDL extensively regulates transcription and alternative splicing

被引：35

作者：

Li, Ruth Zhen ^{[1
,2
]}

Hou, Jing ^{[2
,3
]}

Wei, Yaxun ^{[2
]}

Luo, Xiaotian ^{[3
]}

Ye, Yali ^{[3
]}

Zhang, Yi ^{[2
,3
]}

机构：

[1] Southern Med Univ, Sch Basic Med, Dept Bioinformat, Guangzhou 510515, Guangdong, Peoples R China

[2] ABLife Inc, Ctr Genome Anal, Opt Valley Int Biomed Pk, Bldg 18-1,East Lake High Tech Dev Zone, Wuhan 430075, Hubei, Peoples R China

[3] ABLife Inc, Lab Genome Regulat & Human Hlth, Opt Valley Int Biomed Pk, Bldg 18-2,East Lake High Tech Dev Zone, Wuhan 430075, Hubei, Peoples R China

来源：

GENE | 2019年 / 687卷

关键词：

hnRNPDL; Knock down; RNA-seq; Gene expression; Alternative splicing; PREGNANCY-SPECIFIC BETA-1-GLYCOPROTEIN; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS; TRANSLATIONAL REGULATION; PROTEIN JKTBP; CELL-CYCLE; FACTOR SF1; TGF-BETA; RNA; EXPRESSION; CANCER;

D O I：

10.1016/j.gene.2018.11.026

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

RNA binding proteins (RBPs) are key players of genome regulation. Here we report the transcriptome study of HnRNP D-Like protein, which belongs to the hnRNP family. We used RNA-seq to analyze the global transcript level and alternative splicing on hnRNPDL shRNA-treated cells and control. Sh-hnRNPDL extensively increased in the expression of genes involved in female pregnancy, cell apoptosis, cell proliferation and cell migration. HnRNPDL regulated alternative splicing of hundreds of genes enriched in transcription regulation and signaling pathways including NOD-like receptor signaling, Notch signaling, and TNF signaling. This study provides the first transcriptome-wide analysis of hnRNPDL regulation of gene expression, which adds to the understanding of critical hnRNPDL functions.

引用

页码：125 / 134

页数：10

共 67 条

[1] Molecular characterization of a mouse heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its tissue-specific expression [J].