Increasing mouse embryonic fibroblast cells adhesion on superhydrophilic vertically aligned carbon nanotube films

被引：21
作者：
Lobo, A. O. [1 ,2 ]
Marciano, F. R. [1 ,2 ]
Ramos, S. C. [3 ]
Machado, M. M. [4 ]
Corat, E. J. [3 ]
Corat, M. A. F. [4 ]
机构：
[1] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Nanotechnol NanoBio, BR-12244000 Sao Jose Dos Campos, SP, Brazil
[2] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Vibrat Spect LEVB, BR-12244000 Sao Jose Dos Campos, SP, Brazil
[3] INPE, Lab Associado Sensores & Mat LAS, BR-12245970 Sao Jose Dos Campos, SP, Brazil
[4] Univ Estadual Campinas UNICAMP, Ctr Multidisciplinar Invest Biol, Area Ciencia Anim Lab CEMIB, BR-13083877 Campinas, SP, Brazil
来源：
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2011年 / 31卷 / 07期
基金：
巴西圣保罗研究基金会;
关键词：
VACNTs; Oxygen plasma; Wettability; Superhydrophilic; Cell adhesion; SURFACE FREE-ENERGY; PLASMA TREATMENT; RAMAN-SPECTRA; WETTABILITY; GROWTH; POLYMERS; MECHANOTRANSDUCTION; FUNCTIONALIZATION; BIOCOMPATIBILITY; HYDROPHILICITY;
D O I：
10.1016/j.msec.2011.06.007
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real rime, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：1505 / 1511
页数：7
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