Reversed-phase liquid chromatographic method for the determination of ochratoxin A in wine

被引：49

作者：

Dall'Asta, C ^{[1
]}

Galaverna, G ^{[1
]}

Dossena, A ^{[1
]}

Marchelli, R ^{[1
]}

机构：

[1] Univ Parma, Dipartimento Chim Organ & Ind, I-43100 Parma, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1024卷 / 1-2期

关键词：

wine; mycotoxins; ochratoxin A;

D O I：

10.1016/j.chroma.2003.10.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we propose a new, rapid, highly sensitive and reproducible RP-HPLC-FLD method for the detection of ochratoxin A (OTA) in wine, by directly injecting the liquid in the chromatographic system without any extraction or clean-up. An alkaline mobile phase (NH4Cl:CH3CN 85:15 (v/v), 20 mM, pH 9.8) was used to obtain a distinct fluorescence enhancement. This improvement allows to reach, without an immunoaffinity clean-up or concentration, a detection limit of 0.05 ng/ml, which is similar to those commonly obtained after immunoaffinity purification and acidic elution. The method was statistically validated and directly applied to a series of wine samples. (C) 2003 Elsevier B.V. All fights reserved.

引用

页码：275 / 279

页数：5

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