An injectable alginate/extra cellular matrix (ECM) hydrogel towards acellular treatment of heart failure

被引:49
作者
Curley, Clive J. [1 ,2 ,3 ,4 ]
Dolan, Eimear B. [1 ,2 ,5 ,6 ]
Otten, Matthias [7 ]
Hinderer, Svenja [7 ,8 ]
Duffy, Garry P. [1 ,3 ,4 ,5 ,6 ]
Murphy, Bruce P. [1 ,2 ,3 ,4 ]
机构
[1] Trinity Coll Dublin, Trinity Biomed Sci Inst, Trinity Ctr Bioengn, Dublin 2, Ireland
[2] Trinity Coll Dublin, Dept Mech & Mfg Engn, Sch Engn, Dublin 2, Ireland
[3] Trinity Coll Dublin, Adv Mat & BioEngn Res Ctr AMBER, Dublin, Ireland
[4] Royal Coll Surgeons Ireland, Dublin, Ireland
[5] Royal Coll Surgeons Ireland, Tissue Engn Res Grp, Dept Anat, 123 St Stephens Green, Dublin 2, Ireland
[6] Natl Univ Ireland Galway, Coll Med Nursing & Hlth Sci, Sch Med, Dept Anat, Galway, Ireland
[7] Eberhard Karls Univ Tubingen, Res Inst Womens Hlth, Dept Womens Hlth, Silcherstr 7-1, D-72076 Tubingen, Germany
[8] Univ Tubingen, Nat & Med Sci Inst NMI, Markwiesenstr 55, D-72770 Reutlingen, Germany
关键词
Heart failure; Acellular hydrogel; Minimally invasive delivery catheter; Alginate; Decellularized ECM; MYOCARDIAL-INFARCTION; EXTRACELLULAR-MATRIX; DELIVERY; INJECTION; DRUG; TISSUES;
D O I
10.1007/s13346-018-00601-2
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
As treatments for myocardial infarction (MI) continue to improve, the population of people suffering from heart failure (HF) is rising significantly. Novel treatment strategies aimed at achieving long-term functional stabilisation and improvement in heart function post MI include the delivery of biomaterial hydrogels and myocardial matrix-based therapies to the left ventricle wall. Individually alginate hydrogels and myocardial matrix-based therapies are at the most advanced stages of commercial/clinical development for this potential treatment option. However, despite these individual successes, the potential synergistic effect gained by combining the two therapies remains unexplored. This study serves as a translational step in evaluating the minimally invasive delivery of dual acting alginate-based hydrogels to the heart. We have successfully developed new production methods for hybrid alginate/extracellular matrix (ECM) hydrogels. We have identified that the high G block alginate/ECM hybrid hydrogel has appropriate rheological and mechanical properties (1.6KPa storage modulus, 29KPa compressive modulus and 14KPa dynamic modulus at day 1) and can be delivered using a minimally invasive delivery device. Furthermore, we have determined that these novel hydrogels are not cytotoxic and are capable of enhancing the metabolic activity of dermal fibroblasts in vitro (p<0.01). Overall these results suggest that an effective minimally invasive HF treatment option could be achieved by combining alginate and ECM particles.
引用
收藏
页码:1 / 13
页数:13
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