Granulocyte-colony stimulating factor enhances load-induced muscle hypertrophy in mice

被引：5

作者：

Ohashi, Maiko ^{[1
]}

Okubo, Kazumasa ^{[2
]}

Mizuno, Sakiko ^{[3
]}

Yoda, Masaki ^{[4
]}

Shirasawa, Hideyuki ^{[1
]}

Chiba, Kazuhiro ^{[5
]}

Horiuchi, Keisuke ^{[1
,5
]}

Matsumoto, Morio ^{[1
]}

Nakamura, Masaya ^{[1
]}

机构：

[1] Keio Univ, Dept Orthoped Surg, Sch Med, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan

[2] Sato Pharmaceut Co Ltd, Pharmacol R&D Sect, Pharmaceut Res Dept, Shinagawa Ku, 6-8-5 Higashiohi, Tokyo 1400011, Japan

[3] Tokyo Dent Coll, Dept Orthoped, Ichikawa Gen Hosp, 5-11-13 Sugano, Ichikawa, Chiba 2728513, Japan

[4] Keio Univ, Lab Cell & Tissue Biol, Sch Med, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan

[5] Natl Def Med Coll, Dept Orthoped Surg, 3-2 Namiki, Tokorozawa, Saitama 3598513, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 506卷 / 04期

关键词：

G-CSF; G-CSF receptor; Skeletal muscle; Muscle hypertrophy; SKELETAL-MUSCLE; G-CSF; MYOCARDIAL-INFARCTION; PROLIFERATION; SARCOPENIA; PATHWAY; ATROPHY;

D O I：

10.1016/j.bbrc.2018.10.196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Granulocyte-colony stimulating factor (G-CSF) is a cytokine crucially involved in the regulation of granulopoiesis and the mobilization of hematopoietic stem cells from bone marrow. However, emerging data suggest that G-CSF exhibits more diverse functions than initially expected, such as conferring protection against apoptosis to neural cells and stimulating mitogenesis in cardiomyocytes and skeletal muscle stem cells after injury. In the present study, we sought to investigate the potential contribution of G-CSF to the regulation of muscle volume. We found that the administration of G-CSF significantly enhances muscle hypertrophy in two different muscle overload models. Interestingly, there was a significant increase in the transcripts of both G-CSF and G-CSF receptors in the muscles that were under overload stress. Using mutant mice lacking the G-CSF receptor, we confirmed that the anabolic effect is dependent on the G-CSF receptor signaling. Furthermore, we found that G-CSF increases the diameter of myotubes in vitro and induces the phosphorylation of AKT, mTOR, and ERK1/2 in the myoblast-like cell line C2C12 after differentiation induction. These findings indicate that G-CSF is involved in load-induced muscle hypertrophy and suggest that G-CSF is a potential agent for treating patients with muscle loss and sarcopenia. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：944 / 949

页数：6