Lactiplantibacillusplantarum ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome

被引：15

作者：

Lee, Young-Sil ^{[1
]}

Park, Eun-Jung ^{[2
,3
]}

Park, Gun-Seok ^{[1
]}

Ko, Seung-Hyun ^{[1
]}

Park, Juyi ^{[1
]}

Lee, You-Kyung ^{[1
]}

Kim, Jong-Yeon ^{[2
]}

Lee, Daeyoung ^{[1
]}

Kang, Jihee ^{[1
]}

Lee, Hae-Jeung ^{[2
,3
]}

机构：

[1] AtoGen Co Ltd, Daejeon 34015, South Korea

[2] Gachon Univ, Coll BioNano Technol, Dept Food & Nutr, Seongnam Si 13120, Gyeonggi Do, South Korea

[3] Gachon Univ, Inst Aging & Clin Nutr Res, Seongnam Si 13120, Gyeonggi Do, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 23期

关键词：

obesity; probiotics; Lactiplantibacillus plantarum ATG-K2; gut microbiome; short-chain fatty acids; lipid metabolism; LACTOBACILLUS-GASSERI SBT2055; ADIPOCYTE SIZE; ACIDS; ABSORPTION; BUTYRATE; MASS;

D O I：

10.3390/ijms222312665

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of Lactiplantibacillus plantarum ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of L. plantarum ATG-K2. Mice were fed a normal fat diet or HFD with L. plantarum ATG-K2 and Orlistat for 8 weeks. L. plantarum ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPAR gamma, SREBP1c, and FAS and upregulation of PPAR alpha, CTP1, UCP1, Prdm16, and ND5. Moreover, L. plantarum ATG-K2 decreased TG, T-CHO, leptin, and TNF-alpha levels in the serum, with corresponding gene expression levels in the intestine. L. plantarum ATG-K2 modulated the gut microbiome by increasing the abundance of the Lactobacillaceae family, which increased SCFA levels and branched SCFAs in the feces. L. plantarum ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore, L. plantarum ATG-K2 can be a preventive and therapeutic agent for obesity.

引用

页数：18