Determination of antihypertensive peptides from an izumi shrimp hydrolysate

被引：24

作者：

Nu, Yoshitaka ^{[1
]}

Fukuta, Kazuhiro ^{[1
]}

Yoshimoto, Ryoko ^{[1
]}

Sakai, Kentaro ^{[2
]}

Ogawa, Tadashi ^{[3
]}

机构：

[1] Tokushima Prefectural Ind Technol Ctr, Food Technol Div, Tokushima 7708021, Japan

[2] Hiroshima Jogakuin Univ, Fac Human Life Sci, Dept Nutr & Hlth Promot, Hiroshima 7320063, Japan

[3] Kansai Univ Welf Sci, Dept Nutrit Sci Well Being, Fac Hlth Sci Welf, Osaka 5820026, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 03期

关键词：

angiotensin; peptide; shrimp hydrolysate; anti-hypertension; allergenicity;

D O I：

10.1271/bbb.70565

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The izumi shrimp (Plesionika izumiae Omori, 1971) is an unused resource which can be caught off the southern coast of Tokushima Prefecture. We have previously found that an izumi shrimp hydrolysate significantly inhibited the age-associated spontaneous increase in blood pressure in stroke-prone spontaneously hypertensive rats. In this present study, two angiotensin I-converting enzyme inhibitory peptides were isolated from an izumi shrimp hydrolysate by using high-performance liquid chromatography, and their amino acid sequences were determined to be Val-Trp-Tyr-His-Thr and Val-Trp. A single oral administration of synthetic Val-Trp-Tyr-His-Thr or Val-Trp significantly decreased the blood pressure in stroke-prone spontaneously hypertensive rats. The antigenicity and allergenicity of the izumi shrimp hydrolysate against BALB/c mice were very low. These results demonstrate that the angiotensin I-converting enzyme inhibitory peptides isolated from the izumi shrimp hydrolysate had an anti-hypertensive effect on rats.

引用

页码：861 / 864

页数：4

共 13 条

[1]

CHEUNG HS, 1980, J BIOL CHEM, V255, P401

[2] CONCENTRATIONS OF ANGIOTENSIN-CONVERTING ENZYME IN TISSUES OF RAT [J].

CUSHMAN, DW ;

CHEUNG, HS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 250 (01) :261-+

[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[4] ANGIOTENSIN I-CONVERTING ENZYME-INHIBITORY PEPTIDES IN AN ALKALINE PROTEASE HYDROLYZATE DERIVED FROM SARDINE MUSCLE [J].

MATSUFUJI, H ;

MATSUI, T ;

SEKI, E ;

OSAJIMA, K ;

NAKASHIMA, M ;

OSAJIMA, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (12) :2244-2245

[5] ISOLATION AND CHARACTERIZATION OF ANGIOTENSIN I-CONVERTING ENZYME-INHIBITORY PEPTIDES DERIVED FROM BONITO BOWELS [J].

MATSUMURA, N ;

FUJII, M ;

TAKEDA, Y ;

SHIMIZU, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (10) :1743-1744

[6]

Nii Yoshitaka, 1998, Nippon Shokuhin Kagaku Kogaku Kaishi, V45, P671

[7] Purification of angiotensin I-converting enzyme inhibitors in pelagic thresher Alopias pelagicus muscle hydrolysate and viscera extracts [J].

Nomura, A ;

Noda, N ;

Maruyama, S .

FISHERIES SCIENCE, 2002, 68 (04) :954-956

[8] Inhibition properties of dipeptides from salmon muscle hydrolysate on angiotensin I-converting enzyme [J].

Ono, S ;

Hosokawa, M ;

Miyashita, K ;

Takahashi, K .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2006, 41 (04) :383-386

[9] Angiotensin I-converting enzyme inhibitory peptides derived from wakame (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats [J].

Sato, M ;

Hosokawa, T ;

Yamaguchi, T ;

Nakano, T ;

Muramoto, K ;

Kahara, T ;

Funayama, K ;

Kobayashi, A ;

Nakano, T .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (21) :6245-6252

[10] Antihypertensive effects of Undaria pinnatifida (wakame) peptide on blood pressure in spontaneously hypertensive rats [J].

Suetsuna, K ;

Maekawa, K ;

Chen, JR .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2004, 15 (05) :267-272

← 1 2 →