Structure analysis of a heteropolysaccharide from Taraxacum mongolicum Hand.-Matz. and anticomplementary activity of its sulfated derivatives

被引:57
作者
Chen, MiaoMiao
Wu, Jianjun
Shi, Songshan
Chen, Yonglin
Wang, Huijun
Fan, Hongwei
Wang, Shunchun [1 ]
机构
[1] Shanghai Univ Tradit Chinese Med, MOE Key Lab Standardizat Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China
基金
中国国家自然科学基金;
关键词
Polysaccharide; Taraxacum mongolicum Hand.-Mazz; Sulfated derivative; Structure-activity relationship; Anticomplement; HEPARIN-BINDING SITE; ANTICOAGULANT ACTIVITY; COMPLEMENT-SYSTEM; POLYSACCHARIDES; CHROMATOGRAPHY; ALGAE; ARABINOGALACTAN; LOCALIZATION; IMMUNITY; INHIBIT;
D O I
10.1016/j.carbpol.2016.06.110
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A homogenous water-soluble polysaccharide, DPSW-A, with a deduced chemical structure was extracted from the herb Taraxacum mongolicum Hand.-Mazz. Moreover, 80.813-kDa DPSW-A is composed of three types of monosaccharide, namely rhamnose, arabinose, and galactose, at a molar ratio of 1.0:10.7:11.9. The main chain of DPSW-A contains Terminal-Galp, 1,3-Galp, 1,6-Galp, 1,3,6-Galp, and 1,2,4-Rhap; the branched chain contains Terminal-Araf, 1,5-Araf, and 1,3,5-Araf. The sulfated derivatives prepared from DPSW-A showed inhibitory effects on complement activation through the classical pathway (CH50: Sul-DPSW-A, 3.94 +/- 0.43 mu g/mL; heparin, 104.40 +/- 3.82 mu g/mL) and alternative pathway (AP(50): Sul-DPSW-A, 42.76 +/- 0.46 mu g/mL; heparin, 43.42 +/- 0.22 mu g/mL). Mechanism studies indicated that Sul-DPSW-A inhibited complement activation by blocking C1q, C1r, C1s, and C9, but not C2, C3, C4, and C5. In addition, Sul-DPSW-A displayed limited anticoagulant effects. These results suggest that Sul-DPSW-A prepared from DPSW-A is valuable for treating diseases caused by excessive complement system activation. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:241 / 252
页数:12
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