Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

被引：7
|
作者：
Duan, MR
Ren, H
Mao, P
Wei, CH
Liang, YH
Li, Y [1 ]
Su, XD
机构：
[1] Peking Univ, Coll Life Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
[2] Peking Univ, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Beijing 100871, Peoples R China
[3] Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1750卷 / 01期
基金：
中国国家自然科学基金;
关键词：
WRKY protein; transcription factor; crystallization; X-ray diffraction;
D O I：
10.1016/j.bbapap.2005.03.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 angstrom resolution inhouse and belongs to space group P2(1) with unit-cell parameters a = 64. 10 angstrom, b = 34.88 angstrom, c = 114.72 angstrom, beta = 90.49 degrees. (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：14 / 16
页数：3
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