Diversity of bacteriorhodopsins in different hypersaline waters from a single Spanish saltern

被引:25
作者
Papke, RT [1 ]
Douady, CJ [1 ]
Doolittle, WF [1 ]
Rodríguez-Valera, F [1 ]
机构
[1] Dalhousie Univ, Dept Biochem & Mol Biol, Halifax, NS B3H 4H7, Canada
关键词
D O I
10.1046/j.1462-2920.2003.00501.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Haloarchaeal rhodopsins are a diverse group of transmembrane proteins that use light energy to drive several different cellular processes. Two rhodopsins, bacteriorhodopsin and halorhodopsins, are H+ and Cl- ion pumps, respectively, and two rhodopsins, sensory rhodopsin I and II, regulate phototaxis. Bacteriorhodopsin is of special interest as it is a non-chlorophyll-based type of phototrophy (i.e. generation of chemical energy from light energy). However, very little is known about the diversity and distribution of rhodopsin genes in hypersaline environments. Here, we have used environmental PCR and cloning techniques to directly retrieve rhodopsin genes from three different salinity ponds located in a sea salt manufacturing facility near Alicante, Spain. Our survey resulted in the discovery of previously concealed variation including what is hypothesized to be bacteriorhodopsin genes from the uncultivated square morphotype that dominates these environments. In some instances, identical genes were discovered in seemingly different habitats suggesting that some haloarchaea are present over widely varying concentrations of salt.
引用
收藏
页码:1039 / 1045
页数:7
相关论文
共 35 条
  • [1] Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds
    Antón, J
    Llobet-Brossa, E
    Rodríguez-Valera, F
    Amann, R
    [J]. ENVIRONMENTAL MICROBIOLOGY, 1999, 1 (06) : 517 - 523
  • [2] Coordinate regulation of energy transduction modules in Halobacterium sp analyzed by a global systems approach
    Baliga, NS
    Pan, M
    Goo, YA
    Yi, EC
    Goodlett, DR
    Dimitrov, K
    Shannon, P
    Aebersold, R
    Ng, WV
    Hood, L
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) : 14913 - 14918
  • [3] Quantitative tracing, by Taq nuclease assays, of a Synechococcus ecotype in a highly diversified natural population
    Becker, S
    Fahrbach, M
    Böger, P
    Ernst, A
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (09) : 4486 - 4494
  • [4] Bacterial rhodopsin:: Evidence for a new type of phototrophy in the sea
    Béjà, O
    Aravind, L
    Koonin, EV
    Suzuki, MT
    Hadd, A
    Nguyen, LP
    Jovanovich, S
    Gates, CM
    Feldman, RA
    Spudich, JL
    Spudich, EN
    DeLong, EF
    [J]. SCIENCE, 2000, 289 (5486) : 1902 - 1906
  • [5] Benlloch S, 1996, SYST APPL MICROBIOL, V18, P574
  • [6] The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins
    Bieszke, JA
    Braun, EL
    Bean, LE
    Kang, SC
    Natvig, DO
    Borkovich, KA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (14) : 8034 - 8039
  • [7] Nitrogen-fixing phylotypes of Chesapeake Bay and Neuse River estuary sediments
    Burns, JA
    Zehr, JP
    Capone, DG
    [J]. MICROBIAL ECOLOGY, 2002, 44 (04) : 336 - 343
  • [8] Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern
    Casamayor, EO
    Massana, R
    Benlloch, S
    Ovreås, L
    Díez, B
    Goddard, VJ
    Gasol, JM
    Joint, I
    Rodríguez-Valera, F
    Pedrós-Alió, C
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2002, 4 (06) : 338 - 348
  • [9] Felsenstein J., 2001, PHYLIP PHYLOGENY INF
  • [10] Niche adaptation in ocean cyanobacteria
    Ferris, MJ
    Palenik, B
    [J]. NATURE, 1998, 396 (6708) : 226 - 228