Detection and characterization of Ageratum enation virus and a nanovirus-like satellite DNA1 from zinnia causing leaf curl symptoms in India

被引：12

作者：

Kumar, Yogesh ^{[1
]}

Bhardwaj, Pooja ^{[1
]}

Hallan, Vipin ^{[1
]}

Zaidi, A. A. ^{[1
]}

机构：

[1] CSIR, Inst Himalayan Bioresource Technol, Plant Virus Lab, Floriculture Div, Palampur 176061, Himachal Prades, India

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2010年 / 76卷 / 06期

关键词：

Geminiviridae; Begomovirus; Rolling circle amplification; Phylogenetic analysis; MOLECULAR CHARACTERIZATION; GEMINIVIRUSES; BETA; BEGOMOVIRUSES; DIVERSITY; COMPLEXES; TOMATO; THREAT;

D O I：

10.1007/s10327-010-0270-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Leaf curl in Zinnia elegans was observed in a subtemperate region in northern India, and the causal organism was identified as Ageratum enation virus (AEV). The virus appeared to be monopartite and associated with nanovirus-like satellite DNA1. The whole genome was amplified by rolling circle amplification, cloned, and sequenced using a primer walking strategy. The genome (FN543099) was composed of 2753 nucleotides (nt) and that of DNA1 (FN543100) of 1374 nt. DNA-beta was not detected from any of the infected samples. The study presents the first report of a begomovirus from Zinnia spp. in India and the complete nucleotide sequence characterization of AEV associated with nanovirus-like satellite DNA1.

引用

页码：395 / 398

页数：4